Bacterial Diversity at Different Depths in Lead-Zinc Mine Tailings as Revealed by 16S rRNA Gene Libraries

다국어 초록 (Multilingual Abstract)

Bacterial communities at 10 cm, 100 cm, and 200 cm depths in a 100-year-old lead-zinc tailing heap were
evaluated by constructing 16S rRNA gene libraries. In total, 98 operational taxonomic units (OTUs) were identified from 193 clones at a 3% sequence difference level. The OTU number and species richness decreased with the depth. Species composition was significantly different between the three libraries. Fifty-seven percent
of the examined clones were Acidobacteria and 27% belonged to Proteobacteria. Other sequences included Chloroflexi, Firmicutes, Chlamydiae, Actinobacteria, Gemmatimonadetes, Nitrospira, and three unclassified OTUs. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were mainly distributed in the rhizosphere of naturally colonizing plants; however, Deltaproteobacteria,
Acidobacteria, and Chloroflexi tended to inhabit the deeper tailings (below the 100 cm-depth).

참고문헌 (Reference)

1 "The potential of some soil amendments to improve tree growth on metalliferous mine wastes" 192 : 199-217, 1997

2 "Structure of sediment-associated microbial communities along a heavy-metal contamination gradient in the marine environment" 71 : 679-690, 2005

3 "Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer" 69 : 5884-5891, 2003

4 "Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis" 178 : 5636-5643, 1996

5 "Molecular profiling of rhizosphere microbial communities associated with healthy and diseased black spruce (Picea mariana) seedlings grown in a nursery" 70 : 3541-3551, 2004

6 "Microbiological and geochemical characterization of fluvially deposited sulfidic mine tailings" 65 : 1548-1555, 1999

7 "Microbial populations associated with the generation and treatment of acid mine drainage" 169 : 435-448, 2000

8 "Microbial diversity in uranium mine waste heaps" 61 : 2930-2935, 1995

9 "Introducing DOTUR, a computer grogram for defining operational taxonomic units and estimating species richness" 71 : 1501-1506, 2005

10 "Integron diversity in heavy-metal-contaminated mine tailings and inferences about integron evolution" 70 : 1160-1168, 2004

11 "Integration of microbial ecology and statistics: a test to compare gene libraries" 70 : 5485-5492, 2004

12 "Influence of Typha latifolia and fertilization on metal mobility in two different Pb-Zn mine tailings types" 333 : 9-24, 2004

13 "Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity" 180 : 4765-4774, 1998

14 "Environmental impacts of metal ore mining and processing: A review" 26 : 590-602, 1997

15 "Enumeration of thiobacilliwithin pH-neutral and acidic mine tailings and their role inthe development of secondary mineral soil" 19921904-1912

16 "Effects of heavy metal contamination and remediation on soil microbial communities in the vicinity of a zinc smelter" 27 : 609-617, 1998

17 "Dynamic of heavy metals in a lead-zinc tailings deposited in different years" 22 : 67-69, 2003

18 "Diversity, localization, and physiological properties of filamentous microbes belonging to Chloroflexi subphylum I in mesophilic and thermophilic methanogenic sludge granules" 71 : 7493-7503, 2005

19 "Diversity and characterization of sulfate-reducing bacteria in groundwater at a uranium mill tailings site" 67 : 3149-3160, 2001

20 "Differences in hyporheic-zone microbial community structure along a heavy-metal contamination gradient" 69 : 5563-5573, 2003

21 "Detection of Acidithiobacillus ferrooxidans in acid mine drainage environments using fluorescent in situ hybridization (FISH)" 61 : 33-45, 2005

22 "Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil" 69 : 3223-3230, 2003

23 "Chemical and biological parameters as tools to evaluate and improve heavy metal phytoremediation" 20 : 239-258, 2000

24 "Characterization of bacterial communities in heavy metal contaminated soils" 42 : 593-603, 1996

25 "Change in bacterial community structure during in situ biostimulation of subsurface sediment cocontaminated with uranium and nitrate" 70 : 4911-4920, 2004

26 "Bioremedial potential of microbial mechanisms of metal mobilization and immobilization" 11 : 271-279, 2000

27 "Addition of U(VI) to a uranium mining waste sample and resulting changes in the indigenous bacterial community" 3 : 275-285, 2005