배양한 흰쥐 대뇌세포의 저산소증모델에서 蘇合香元이 유전자 표현에 미치는 영향 = Effects of Sohaphyang-won on the gene expression in a hypoxic model of cultured rat cortical cells

다국어 초록 (Multilingual Abstract)

The purpose of this investigation is to evaluate the effects of Sohaphyang-won(SH) on the alteration in gene expression in a hypoxia model using cultured rat cortical cells. E18 rat cortical cells were grown in Neurobasal medium containing B27 supplement. On 12 DIV, SH was added (20㎍/㎖) to the culture media for 24 hrs. On 14 DIV, cells were given a hypoxic insult (2% O_(2)/5% CO_(2), 37°C, 3 hrs), returned to normoxia and culture for another 24 hrs. Total RNA was prepared from SH-untreated (control) and -treated cultures and alteration in gene expression was analyses by microarray using rat 5K-TwinChips.
Effects on some of the genes whose functions are implicated in neural viability are as follows:
1) For most of the genes altered in expression, the Global M values were between -0.5 to +0.5. Among these, 1517 genes were increased in their expression by more than Global M +0.1, while 1480 genes were decreased by more than Global M -0.1.
2) The expression of apoptosis-related genes such as Bad (Global M = 0.35), tumor protein p53 (T53) (Global M = 0.28) were increased, while v-akt murine thymoma viral oncogene homolog 1(Akt1) was decreased.
3) The expression of hemoglobin alpha 1 (probably neuroglobin) was increased by about 3.2-fold (Global M = 1.7).
4) The expression of antioxidation-related catalase gene was increased (Global M = 0.26).
5) The expression of PKCzeta(prkcz), an upstream kinase of MAPK, was increased (Global M = 0.29).
6) The expression of retinoic acid receptor alpha (RARα), which may regulated transcription in hypoxic stress, was increased (Global M = 10.27).
In summary, the microarray data suggest that SH doesn't increase the expression of oxygen capture-, anti-oxidation- and 'Response to stress'-related genes but also decreases some anti-apoptosis genes which would help protect the hypoxiv cells from apoptosis.

목차 (Table of Contents)

• 目次
• Ⅰ. 緖論 = 1
• Ⅱ. 實驗 = 2
• 1. 재료 = 2
• 1) 약재 = 2
• 2) 蘇合香元 물추출액 제조 = 2
• 2. 방법 = 3
• 1) 신경세포 배양 = 3
• 2) 저산소증 모델 = 3
• 3) 蘇合香元 물추출액의 처리 = 4
• 4) RNA 추출 = 4
• 5) Microarray = 4
• Ⅲ. 結果 = 5
• 1. microarray 결과 = 5
• 2. Apoptosis 관련 유전자 표현의 변화 = 5
• 3. 세포의 성장과 유지에 관련된 유전자 표현의 변화 = 6
• 4. 세포주기 관련 유전자 표현의 변화 = 6
• 5. 스트레스 반응 관련 유전자 표현의 변화 = 6
• 6. 신호전달 관련 유전자 표현의 변화 = 6
• 7. 전사(transcription) 관련 유전자 표현의 변화 = 6
• Ⅳ. 考察 = 21
• Ⅴ. 結論 = 25
• 參考文獻 = 26
• ABSTRACT = 31