Botrytis cinerea에 의한 오크라 잿빛곰팡이병 = Occurrence of Gray Mold Caused by Botrytis cinerea on Okra in Korea

다국어 초록 (Multilingual Abstract)

From 2014 to 2016, approximately 5% of okra fruit were observed displaying gray mold symptoms at the research field of Jeollabuk-do Agricultural Research and Extension Services, Korea. The symptoms observed were water-soaked, brown or gray spots, and abundant mycelial with conidia appearing on the infected fruit.
Initial infection commenced from the base of fruit and gradually moved to the pod, where it finally resulted in collapse. Colonies on potato dextrose agar were gray to grayish brown, felted and cottony expanding 65-80 mm after one week. The fungus formed several black sclerotia ranging 1.0-3.5×0.5-3.0 mm on the Petri dish after two weeks. The conidia were one-celled, ellipsoidal or ovoid, colorless or pale brown, and 6.2-15.4×5.0- 10.4 mm. Conidiophores arose solitary or in groups, straight or flexuous, septate, with an inflated basal cell brown to light brown, and measured 85-450×10.0-40.0 mm. On the basis of the morphological characteristics and phylogenetic analyses of internal transcribed spacer rDNA, the fungus was identified as Botrytis cinerea Pers. Pathogenicity of a representative isolate was proved by artificial inoculation, fulfilling Koch’s postulates.
To our knowledge, this is the first report on the occurrence of B. cinerea on okra in Korea.

참고문헌 (Reference)

1 ten Have, A., "The Botrytis cinerea aspartic proteinase family" 47 : 53-65, 2010

2 Ministry of Agriculture, Food and Rural Affairs, "Statistics of cultivation and production of industrial crops in 2015" Korean Ministry of Agriculture, Food and Rural Affairs 2015

3 White, T. J., "PCR - Protocols and Applications - A Laboratory Manual" Academic Press 315-322, 1990

4 Tamura, K., "MEGA6: molecular evolutionary genetics analysis version 6.0" 30 : 2725-2729, 2013

5 The Korean Society of Plant Pathology, "List of plant disease in Korea" The Korean Society of Plant Pathology 853-, 2009

6 Salameh, N., "Genetic diversity of okra (Abelmoschus esculentus L.) landraces from different agro-ecological regions revealed by AFLP analysis" 14 : 155-160, 2014

7 Farr, D. F, "Fungal Databases, Systematic Mycology & Microbiology Laboratory"

8 Choi, I. Y., "First report of powdery mildew caused by Podosphaera xanthii on okra in Korea" 102 : 1663-, 2018

9 Zhao, Q., "First report of grey mould disease on Abelmoschus esculentus caused by Botrytis cinerea in China" 164 : 354-357, 2016

10 Aktaruzzaman, M., "First report of gray mold caused by Botrytis cinerea on strawflower in Korea" 102 : 452-, 2018

11 Park, J. H., "First report of Choanephora rot of okra caused by Choanephora cucurbitarum in Korea" 163 : 503-506, 2015

12 FAOSTAT, "FAOSTAT URL"

13 Ellis, M., "Dematiaceous Hyphomycetes" Commonwealth Mycological Institute 608-, 1971

14 Plant Disease Diagnostic Clinic, "Botrytis blight: Botrytis cinerea;Botrytis spp.. Cornell University, Department of Plant Pathology and Plant-Microbe Biology Report"

15 Mishra, G. P., "Biotechnological advancements and begomovirus management in okra (Abelmoschus esculentus L.): status and perspectives" 8 : 360-, 2017