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      Characterization of Protease-Producing and Organic Acid-Producing Bacteria for Chitin Production from Mealworm

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      https://www.riss.kr/link?id=A108780314

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      다국어 초록 (Multilingual Abstract)

      Bacillus subtilis EG1 and Leuconostoc mesenteroides BW2 were isolated for use as protease- and lipaseproducing bacteria, respectively. Bacillus subtilis EG1 strain was confirmed to have strong proteolytic activity after 45 h of culturing on a skim milk plate. The culture medium inoculated with B. subtilis EG1 had relatively higher protein resolution and showed many more emulsion forms than that inoculated with L. mesenteroides BW2. The pH of the culture medium with B. subtilis EG1 gradually increased to 6.83, whereas that of the culture medium with L.
      mesenteroides BW2 decreased to 3.6 due to organic acid production. On the initial day, the soluble peptide contents of the culture media with B. subtilis EG1 and L. mesenteroides BW2 were 0.65 µmol/mL and 0.56 µmol/mL, respectively. However, just 3 d after culture, the soluble peptide content of the media with B. subtilis EG1 increased to 2.0 µmol/mL. The lipase activity of B. subtilis EG1 reached a maximum value of 24.6 units/mg protein on the first day of incubation in a mealworm medium. The proteases of P1, P2, and P9 isozymes of B. subtilis EG1 were the major active enzymes.
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      Bacillus subtilis EG1 and Leuconostoc mesenteroides BW2 were isolated for use as protease- and lipaseproducing bacteria, respectively. Bacillus subtilis EG1 strain was confirmed to have strong proteolytic activity after 45 h of culturing on a skim mil...

      Bacillus subtilis EG1 and Leuconostoc mesenteroides BW2 were isolated for use as protease- and lipaseproducing bacteria, respectively. Bacillus subtilis EG1 strain was confirmed to have strong proteolytic activity after 45 h of culturing on a skim milk plate. The culture medium inoculated with B. subtilis EG1 had relatively higher protein resolution and showed many more emulsion forms than that inoculated with L. mesenteroides BW2. The pH of the culture medium with B. subtilis EG1 gradually increased to 6.83, whereas that of the culture medium with L.
      mesenteroides BW2 decreased to 3.6 due to organic acid production. On the initial day, the soluble peptide contents of the culture media with B. subtilis EG1 and L. mesenteroides BW2 were 0.65 µmol/mL and 0.56 µmol/mL, respectively. However, just 3 d after culture, the soluble peptide content of the media with B. subtilis EG1 increased to 2.0 µmol/mL. The lipase activity of B. subtilis EG1 reached a maximum value of 24.6 units/mg protein on the first day of incubation in a mealworm medium. The proteases of P1, P2, and P9 isozymes of B. subtilis EG1 were the major active enzymes.

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      참고문헌 (Reference)

      1 Sadler, G. D., "pH and titratable acidity" 4 : 219-238, 2010

      2 Cupp-Enyard, C., "Sigma's non-specific protease activity assay-casein as a substrate" (19) : e899-, 2008

      3 Kumar, D., "Screening, isolation and production of lipase/esterase producing Bacillus sp. strain DVL2 and its potential evaluation in esterification and resolution reactions" 4 (4): 1763-1770, 2012

      4 Lesuisse, E., "Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme" 216 (216): 155-160, 1993

      5 Lesuisse, E., "Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme" 216 (216): 155-160, 1993

      6 Eko Sukohidayat, N. H., "Purification and characterization of lipase produced by Leuconostoc mesenteroides subsp. mesenteroidesATCC 8293 using an aqueous two-phase system (ATPS) composed of triton X-100 and maltitol" 23 (23): 1800-, 2018

      7 Sánchez-González, M., "Proteolytic processing of dextransucrase of Leuconostoc mesenteroides" 181 (181): 25-30, 1999

      8 Sánchez-González, M., "Proteolytic processing of dextransucrase of Leuconostoc mesenteroides" 181 (181): 25-30, 1999

      9 Pant, G., "Production, optimization and partial purification of protease from Bacillus subtilis" 9 (9): 50-55, 2015

      10 Jung, W. J., "Production of chitin from red crab shell waste by successive fermentation with Lactobacillus paracasei KCTC-3074 and Serratia marcescens FS-3" 68 (68): 746-750, 2007

      1 Sadler, G. D., "pH and titratable acidity" 4 : 219-238, 2010

      2 Cupp-Enyard, C., "Sigma's non-specific protease activity assay-casein as a substrate" (19) : e899-, 2008

      3 Kumar, D., "Screening, isolation and production of lipase/esterase producing Bacillus sp. strain DVL2 and its potential evaluation in esterification and resolution reactions" 4 (4): 1763-1770, 2012

      4 Lesuisse, E., "Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme" 216 (216): 155-160, 1993

      5 Lesuisse, E., "Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme" 216 (216): 155-160, 1993

      6 Eko Sukohidayat, N. H., "Purification and characterization of lipase produced by Leuconostoc mesenteroides subsp. mesenteroidesATCC 8293 using an aqueous two-phase system (ATPS) composed of triton X-100 and maltitol" 23 (23): 1800-, 2018

      7 Sánchez-González, M., "Proteolytic processing of dextransucrase of Leuconostoc mesenteroides" 181 (181): 25-30, 1999

      8 Sánchez-González, M., "Proteolytic processing of dextransucrase of Leuconostoc mesenteroides" 181 (181): 25-30, 1999

      9 Pant, G., "Production, optimization and partial purification of protease from Bacillus subtilis" 9 (9): 50-55, 2015

      10 Jung, W. J., "Production of chitin from red crab shell waste by successive fermentation with Lactobacillus paracasei KCTC-3074 and Serratia marcescens FS-3" 68 (68): 746-750, 2007

      11 Hassan, M. A., "Production and characterization of keratinolytic protease from new wool-degrading Bacillus species isolated from Egyptian ecosystem" 2013 : 175012-, 2013

      12 Bautista, J., "Preparation of crayfish chitin by in situ lactic acid production" 37 (37): 229-234, 2001

      13 Kumar, S., "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets" 33 (33): 1870-1874, 2016

      14 De Paula, A. T., "Leuconostoc mesenteroides SJRP55: a potential probiotic strain isolated from Brazilian water buffalo mozzarella cheese" 65 : 899-910, 2015

      15 Blásquez, J. R. -E., "In Could grasshoppers be a nutritive meal" 3 : 164-175, 2012

      16 Khusniati, T., "In Characteristic of indigenous Leuconostoc mesenteroides EN 17-11 protease and its stability during storage at cold and freezing temperatures" AIP Publishing 2022

      17 Benson, D. A, "GenBank" 41 : D36-42, 2013

      18 Bourdichon, F., "Food fermentations: microorganisms with technological beneficial use" 154 (154): 87-97, 2012

      19 송용수 ; 김민우 ; 문채영 ; 서동준 ; 한연수 ; 조용훈 ; 노미영 ; 박영규 ; 김선암 ; 김영욱 ; 정우진, "Extraction of chitin and chitosan from larval exuvium and whole body of edible mealworm, Tenebrio molitor" 한국곤충학회 48 (48): 227-233, 2018

      20 Eggert, T., "Extracellular lipases from Bacillus subtilis: regulation of gene expression and enzyme activity by amino acid supply and external pH" 225 (225): 319-324, 2003

      21 Jukes, T. H., "Evolution of protein molecules" 3 : 21-132, 1969

      22 Ciurko, D., "Enzymatichydrolysis using bacterial cultures as a novel method for obtaining antioxidant peptides from brewers' spent grain" 11 (11): 4688-4700, 2021

      23 Heussen, C., "Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates" 102 (102): 196-202, 1980

      24 Tang, C., "Edible insects as a food source: a review" 1 (1): 1-13, 2019

      25 Diaz, P., "Direct fluorescence-based lipase activity assay" 27 (27): 696-700, 1999

      26 정은지 ; 문대원 ; 오준석 ; 문진석 ; 성현빈 ; 김광엽 ; 한남수, "Development of Cabbage Juice Medium for Industrial Production of Leuconostoc mesenteroides Starter" 한국미생물·생명공학회 27 (27): 2112-2118, 2017

      27 Iqbal, S. A., "Characterization of lipase from Bacillus subtilis I-4 and its potential use in oil contaminated wastewater" 58 : 789-797, 2015

      28 Bracco, P., "Bacillus subtilis lipase A-Lipase or esterase?" 10 (10): 308-, 2020

      29 Bradford, M. M., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 (72): 248-254, 1976

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