C57BL/6 마우스에서 치약의 모발성장 촉진 효과: 유효 성분과 유전체 발현에 미치는 영향 = Hair growth promoting effect of toothpaste in C57BL/6 mice: Active components and their effects on genomic expression

다국어 초록 (Multilingual Abstract)

It has been suggested that some toothpastes have the potential to promote hair growth. However, there was no scientific verification on the hair growth effect of toothpaste and no scientific report on major active ingredients in toothpaste. In this work, toothpaste and its constituents were applied topically over the shaved skin of C57BL/6 mice and evaluated. Results indicated that toothpaste showed hair growth effect. Also, the effect of toothpaste constituents on the proliferation rate of keratinocyte cells was investigated. The mixture solution of α-tocopherol acetate, l-menthol, and stevioside, each of that was known to promote hair growth and other toothpaste constituents were applied topically on mouse skin. When the mixture solution was included, hair growth effect was observed in mice. Transcriptome analysis was performed using the dorsal epidermis of mice from the group treated with toothpaste, the mixture which are presumed to be active ingredients for hair growth, and from mice used for the control group. As a result of analyzing the genes whose expression was significantly changed in each treatment group, the gene patterns of the two groups were very similar. Also, when functional genomic analysis was performed, genes with functions related to hair growth regulation showed a high extent of the change in both groups. Hair growth-related genes whose expression was changed in both groups included keratin, keratinrelated proteins, forkhead box, and sonic hedgehog. Therefore, the hair growth effect of toothpaste is thought to be due to the effect of a mixture of α-tocopherol acetate, l-menthol, and stevioside.

참고문헌 (Reference)

1 Goralczyk, "inventor" DSM NUTRITIONAL PRODUCTS LTD 2013

2 Chao-Chun Yang, "Review of hair follicle dermal cells" Elsevier BV 57 (57): 2-11, 2010

3 오지영, "Peppermint Oil Promotes Hair Growth without Toxic Signs" 한국독성학회 30 (30): 297-304, 2014

4 Rachel Sennett, "Mesenchymal–epithelial interactions during hair follicle morphogenesis and cycling" Elsevier BV 23 (23): 917-927, 2012

5 Ralf Paus, "In search of the “hair cycle clock”: a guided tour" Elsevier BV 72 (72): 489-511, 2004

6 Y.-S. Su, "Icariin promotes mouse hair follicle growth by increasing insulin-like growth factor 1 expression in dermal papillary cells" Wiley 42 (42): 287-294, 2017

7 Ben Langmead, "Fast gapped-read alignment with Bowtie 2" Springer Science and Business Media LLC 9 (9): 357-359, 2012

8 Jungyoon Ohn, "Evaluating hair growth promoting effects of candidate substance: A review of research methods" Elsevier BV 93 (93): 144-149, 2019

9 Beoy LA, "Effects of tocotrienol supplementation on hair growth in human volunteers" 21 : 91-99, 2010

10 Katherine Elliott, "Differences in Hair Follicle Dermal Papilla Volume are Due to Extracellular Matrix Volume and Cell Number: Implications for the Control of Hair Follicle Size and Androgen Responses" Elsevier BV 113 (113): 873-877, 1999

11 Woo Chi, "Dermal papilla cell number specifies hair size, shape and cycling and its reduction causes follicular decline" The Company of Biologists 140 (140): 1676-1683, 2013

12 K. S. Stenn, "Controls of Hair Follicle Cycling" American Physiological Society 81 (81): 449-494, 2001

13 Anna-Marie Hosking, "Complementary and Alternative Treatments for Alopecia: A Comprehensive Review" S. Karger AG 5 (5): 72-89, 2019

14 Robert C Gentleman, "Bioconductor : open software development for computational biology and bioinformatics" Springer Science and Business Media LLC 5 (5): R80-, 2004

15 Aaron R. Quinlan, "BEDTools: a flexible suite of utilities for comparing genomic features" Oxford University Press (OUP) 26 (26): 841-842, 2010

16 Sven Müller-Röver, "A Comprehensive Guide for the Accurate Classification of Murine Hair Follicles in Distinct Hair Cycle Stages" Elsevier BV 117 (117): 3-15, 2001