리포솜 이중층의 stigmasterol이 포집된 ascorbic acid의 안정성에 미치는 영향

국문 초록 (Abstract)

SS은 식물성 스테롤로서 인체에 유익한 생리활성을 나타낸다. 리포솜 인지질을 PC과 SS로 구성하고, ASA를 탈수/재수화 방법에 의해 포집시킨 후, ASA의 안정성을 분석하였다. 리포솜의 평균 크기는 SS 함량이 증가할수록 증가하였으며, SS는 포집된 ASA의 안정성을 향상시켰다. 예를 들어, 완충용액에 존재하는 ASA는 4℃에서 8일간 저장하면 7.66%가 잔존하지만, 100:0, 90:10, 70:30 (PC/SS)로 조성된 리포솜에 포집된 ASA는 같은 조건에서 각각 34.12, 49.88, 58.58%가 잔존하였다. 이러한 결과는 리포솜에서 SS가 포집된 ASA의 안정성을 향상시킴을 의미한다.

다국어 초록 (Multilingual Abstract)

The effect of stigmasterol (SS), a phytosterol, in the liposome bilayer on the stability of encapsulated ascorbic acid (ASA) was evaluated. Liposomes, consisting of phosphatidylcholine (PC) and SS, and ASA were encapsulated by the dehydration/rehydration method. The average particle size of the liposome increased with increasing SS content. SS significantly increased the stability of encapsulated ASA. For example, ASA remaining in the liposomes of 100:0, 90:10, and 70:30 (PC:SS, w/w) ratios was 34.12%, 49.88%, and 58.58%, respectively, after storage for 8 days at 4℃, while only 7.66% ASA remained in the buffer under the same conditions. These results indicated that SS in liposomes increased the stability of encapsulated ASA.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• References

참고문헌 (Reference)

1 Lee SC, "The effect of cholesterol in the liposome bilayer on the stabilization of incorporated retinol" 15 : 157-166, 2005

2 Johnson SM, "The effect of charge and cholesterol on the size and thickness of sonicated phospholipid vesicles" 307 : 27-41, 1973

3 이승철, "Stabilization of RetinolGthrough Incorporation into Liposomes" 한국생화학분자생물학회 35 (35): 358-363, 2002

4 Cabral CE, "Phytosterols in the treatment of hypercholesterolemia and prevention of cardiovascular diseases" 109 : 475482-, 2017

5 Koynova R, "Phases and phase transitions of the phosphatidylcholines" 1376 : 91-145, 1998

6 Beutler HO, "Methods in Enzymatic Analysis" Verlag Chemie 376-385, 1984

7 Tran VV, "Liposomes for delivery of antioxidants in cosmeceuticals : Challenges and development strategies" 300 : 114-140, 2019

8 Emami S, "Liposomes as carrier vehicles for functional compounds in food sector" 11 : 737-759, 2016

9 Akbarzadeh A, "Liposome : classification, preparation, and applications" 8 : 102-, 2013

10 Coderch L, "Influence of cholesterol on liposome fluidity by EPR. Relationship with percutaneous absorption" 68 : 85-95, 2000

11 Herbig AL, "Factors that impact the stability of vitamin C at intermediate temperature in a food matrix" 220 : 444-451, 2017

12 Lee YW, "Effect of liposome on the stabilization of ascorbic acid" 31 : 280-284, 1999

13 de Meyer F, "Effect of cholesterol on the structure of a phospholipid bilayer" 106 : 3654-3658, 2009

14 Rhim CH, "Effect of cholesterol in liposome on the stabilization of encapsulated ascorbic acid" 42 : 205-209, 1999

15 Kratz M, "Dietary cholesterol, atherosclerosis and coronary heart disease" 170 : 195-213, 2005

16 Kirby CJ, "Dehydration-rehydration vesicles, a simple method for high yield drug entrapment in liposome" 2 : 979-984, 1984

17 Arrigoni O, "Ascorbic acid : much more than just an antioxidant, Biochim" 1569 : 1-9, 2002

18 Daraee H, "Application of liposomes in medicine and drug delivery" 44 : 381-391, 2016