Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus

다국어 초록 (Multilingual Abstract)

Objectives: Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus. Materials and Methods: Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30). The antibacterial properties of composite resin discs were evaluated by direct contact test. Diluted solutions of Streptococcus mutans (PTCC 1683) and Lactobacillus (PTCC 1643) were prepared. 0.01 mL of each bacterial species was separately placed on the discs. The discs were transferred to liquid culture media and were incubated at 37℃ for 8 hr. 0.01 mL of each solution was cultured on blood agar and the colonies were counted. Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests. Results: Composites containing nano zinc- oxide particles or silver nanoparticles exhibited higher antibacterial activity against Streptococcus mutans and Lactobacillus compared to the control group (p < 0.05). The effect of zinc-oxide on Streptococcus mutans was significantly higher than that of silver (p < 0.05). There were no significant differences in the antibacterial activity against Lactobacillus between composites containing silver nanoparticles and those containing zinc-oxide nanoparticles. Conclusions: Composite resins containing silver or zinc- oxide nanoparticles exhibited antibacterial activity against Streptococcus mutans and Lactobacillus.

참고문헌 (Reference)

1 Cummins D., "Zinc citrate/Triclosan: a new anti-plaque system for the control of plaque and the prevention of gingivitis: short-term clinical and mode of action studies" 18 : 455-461, 1991

2 Sahoo SK, "The present and future of nanotechnology in human health care" 3 : 20-31, 2007

3 Hernández-Sierra JF, "The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold" 4 : 237-240, 2008

4 Bürgers R, "The anti-adherence activity and bactericidal effect of microparticulate silver additives in composite resin materials" 54 : 595-601, 2009

5 Xu T, "Tetrapod-like nano-particle ZnO/acrylic resin composite and its multi-function property" 46 : 297-301, 2003

6 Martinez-Gutierrez F, "Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles" 6 : 681-688, 2010

7 Li LH, "Synthesis and characterization of chitosan/ZnO nanoparticle composite membranes" 345 : 994-998, 2010

8 Beyth N, "Surface antimicrobial activity and biocompatibility of incorporated polyethylenimine nanoparticles" 29 : 4157-4163, 2008

9 Sondi I, "Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria" 275 : 177-182, 2004

10 Rai M, "Silver nanoparticles as a new generation of antimicrobials" 27 : 76-83, 2009

11 Kumar R, "Silver ion release from antimicrobial polyamide/silver composites" 26 : 2081-2088, 2005

12 Wiegand A, "Review on fluoridereleasing restorative materials-fluoride release and uptake characteristics, antibacterial activity and influence on caries formation" 23 : 343-362, 2007

13 Li LH, "Preparation, characterization and antimicrobial activities of chitosan/Ag/ZnO blend films" 160 : 378-382, 2010

14 Melo MA, "Novel dental adhesives containing nanoparticles of silver and amorphous calcium phosphate" 29 : 199-210, 2013

15 Svanberg M, "Mutans streptococci in plaque from margins of amalgam, composite, and glassionomer restorations" 69 : 861-864, 1990

16 Sheng J, "Multi-target antimicrobial actions of zinc against oral anaerobes" 50 : 747-757, 2005

17 김지선, "Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin" 대한치과보존학회 38 (38): 36-42, 2013

18 Tanagawa M, "Inhibitory effect of antibacterial resin composite against Streptococcus mutans" 33 : 366-371, 1999

19 김수현, "Inhibition of Streptococcus mutans biofilm formation on composite resins containing ursolic acid" 대한치과보존학회 38 (38): 65-72, 2013

20 Papagiannoulis L, "In vivo vs in vitro anticariogenic behavior of glass-ionomer and resin composite restorative materials" 18 : 561-569, 2002

21 Thomé T, "In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries" 37 : 705-711, 2009

22 van Dijken JW, "Fluoride and mutans streptococci levels in plaque on aged restorations of resin-modified glass ionomer cement, compomer and resin composite" 31 : 379-383, 1997

23 Pandit S, "Evaluation of Streptococcus mutans biofilms formed on fluoride releasing and non fluoride releasing resin composites" 39 : 780-787, 2011

24 Applerot G, "Coating of glass with ZnO via ultrasonic irradiation and a study of its antibacterial properties" 256 : S3-S8, 2009

25 Schmidlin PR, "Bonding of brackets using a caries-protective adhesive patch" 36 : 125-129, 2008

26 Spencer CG, "Antimicrobial effects of zinc oxide in an orthodontic bonding agent" 79 : 317-322, 2009

27 Kim JS, "Antimicrobial effects of silver nanoparticles" ELSEVIER SCIENCE BV 3 (3): 95-101, 2007

28 Spacciapoli P, "Antimicrobial activity of silver nitrate against periodontal pathogens" 36 : 108-113, 2001

29 Tavassoli Hojati S, "Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles" 29 : 495-505, 2013

30 Imazato S., "Antibacterial properties of resin composites and dentin bonding systems" 19 : 449-457, 2003

31 Pereira-Cenci T, "Antibacterial agents in composite restorations for the prevention of dental caries" 8 : CD007819-, 2009

32 Yoshida K, "Antibacterial activity of resin composites with silvercontaining materials" 107 : 290-296, 1999

33 Pinto RJ, "Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers" 5 : 2279-2289, 2009

34 Aydin Sevinç B, "Antibacterial activity of dental composites containing zinc oxide nanoparticles" 94 : 22-31, 2010

35 Sadowsky SJ., "An overview of treatment considerations for esthetic restorations: a review of the literature" 96 : 433-442, 2006

36 Bundy KJ, "An investigation of the bacteriostatic properties of pure metals" 14 : 653-663, 1980

37 Opdam NJ, "A retrospective clinical study on longevity of posterior composite and amalgam restorations" 23 : 2-8, 2007