Overexpression and Inactivation of UGT73B2 Modulate Tolerance to Oxidative Stress in Arabidopsis

다국어 초록 (Multilingual Abstract)

Glycosyltransferases (GTs) play an important role in modulating solubility, stability, bioavailability, and bioactivity of secondary metabolites, such as flavonoids. In Arabidopsis thaliana, at least 120 family 1 uridine diphosphate (UDP)-glycosyltransferases (UGTs) have been predicted. However, little is known about their substrates or their physiological roles in planta. To define the role of UGT73B2 in planta, we first characterized its expression pattern using transgenic Arabidopsis plants carrying the ciselements of UGT73B2 fused to the GUS reporter. During vegetative phase, its expression was high in embryonic and postembryonic roots, where it may play a physiological role in the glycosylation of flavonoids. Loss of function of UGT73B2 alone or in conjunction with its closest homologs,UGT73B1 and UGT73B3, confers greater tolerance to oxidative stress, whereas overexpression of UGT73B2increases sensitivity to oxidative stress. In addition, growth phenotypes of mutant and transgenic seedlings correlate well with ROS levels in planta. Our results suggest that the glycosylation of flavonoids by UGT73B2—and/or its closest homologs—modulate the response of plants to oxidative stress.

참고문헌 (Reference)

1 Hanasaki Y, "The correlation between active oxygens scavenging and antioxidative effects of flavonoids" 16 : 845-850, 1994

2 Bowler C, "Superoxide dismutases and stress tolerance" 43 : 83-116, 1992

3 Rice-Evans CA, "Structure-antioxidant activity relationships of flavonoids and phenolic acids" 20 : 933-956, 1996

4 Dixon RA, "Stress-induced phenylpropanoid metabolism" 7 : 1085-1097, 1995

5 Cotelle N, "Scavenger and antioxidant properties of ten synthetic flavones" 13 : 211-219, 1992

6 Asada K, "Production and scanvenging of active oxygen in Photosynthesis. in : Photoinhibition" Elsevier 227-287, 1987

7 Gachon CMM, "Plant secondary metabolism glycosyltransferases: the emerging functional analysis" 10 : 542-549, 2005

8 Li Y, "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana" 276 : 4338-4343, 2001

9 Zhou YC, "Phenolic compounds and an analogue as superoxide anion scavengers and antioxidants" 42 : 1177-1179, 1991

10 Langlois-Meurinne M, "Pathogenresponsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis" 139 : 1890-1901, 2005

11 Blanvillain R, "Oxidative stress 3 is chromatin-associated factor involved in tolerance to heavy metals and oxidative stress" 57 : 654-665, 2009

12 Paquette S, "On the origin of family 1 plant glycosyltransferases" 62 : 399-413, 2003

13 Kim JH, "Molecular cloning, expression, and characterization of a flavonoid glycosyltransferase from Arabidopsis thaliana" 170 : 897-903, 2006

14 Chae Eun Lim, "Molecular Genetic Analysis of Tandemly Located Glycosyltransferase Genes, UGT73B1, UGT73B2, and UGT73B3, in Arabidopsis thaliana" 한국식물학회 49 (49): 309-314, 2006

15 Hemm MR, "Light induces phenylpropanoid metabolism in Arabidopsis roots" 38 : 765-778, 2004

16 Lee MH, "Largescale analysis of the GRAS gene family in Arabidopsis thaliana" 67 : 659-670, 2008

17 Achard P, "Integration of plant responses to environmentally activated phytohormonal signals" 311 : 91-94, 2006

18 Chae Eun Lim, "Improved Resistance to Oxidative Stress by a Loss-of-Function Mutation in the Arabidopsis UGT71C1 Gene" 한국분자세포생물학회 25 (25): 368-375, 2008

19 Ross J, "Higher plant glycosyltransferases" 2 : 3004.1-3004.6, 2001

20 Jones P, "Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers" 213 : 164-174, 2001

21 Vogt T, "Glycosyltransferase in plant natural product synthesis: characterization of a supergene family" 5 : 380-386, 2000

22 Earley KW, "Gateway-compatible vectors for plant functional genomics and proteomics" 45 : 616-629, 2006

23 Clough S, "Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana" 16 : 735-743, 1998

24 Yuting C, "Flavonoids as superoxide scavengers and antioxidants" 9 : 19-21, 1990

25 Jovanovic SV, "Flavonoids as antioxidants" 116 : 4846-4851, 1994

26 Chong J, "Downregulation of a pathogen-responsive tobacco UDP-Glc: phenylpropanoid glucosyltransferase reduces scopoletin glucoside accumulation, enhances oxidative stress, and weakens virus resistance" 14 : 1093-1107, 2002

27 Suh MC, "Cadmium resistance in transgenic tobacco plants expressing the Nicotiana glutinosa L. metallothionein-like gene" 8 : 678-684, 1998

28 Heller W, "Biosynthesis of flavonoids. in : The flavonoids: advances in research since 1986" Chapman & Hall 499-536, 1994

29 Li J, "Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation" 5 : 171-179, 1993

30 Mylona PV, "Antioxidant gene responses to ROS-generating xenobiotics in developing and germinated scutella of maize" 58 : 1301-1312, 2007

31 Lim E-K, "Activity of the group 1 glycosyltransferase of Arabidopsis towards salicylic acid, para-hydroxybenzoic acid and other benzoates" 277 : 586-592, 2002

32 Bowles D, "A multigene family of glycosyltransferases in a model plant, Arabidopsis thaliana" 30 : 301-306, 2002