Lactobacillus plantarum Lipoteichoic Acid Alleviates TNF-alpha-Induced Inflammation in the HT-29 Intestinal Epithelial Cell Line

다국어 초록 (Multilingual Abstract)

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-me-diated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF- also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF- stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

참고문헌 (Reference)

1 Neuhaus, F. C., "continuum of anionic charge: structures and functions of D-alanyl-teichoic acids in Gram-positive bacteria" 67 : 686-723, 2003

2 Narayanan, P., "Tumor necrosis factor-α signaling in macrophages" 20 : 87-103, 2010

3 Sheikh, S. Z., "The role of the macrophage in sentinel responses in intestinal immunity" 26 : 578-582, 2010

4 Cassatella, M. A., "The production of cytokines by polymorphonuclear neutrophils" 16 : 21-26, 1995

5 Rescigno, M, "The intestinal epithelial barrier in the control of homeostasis and immunity" 32 : 256-264, 2011

6 Waugh, D. J. J., "The interleukin-8 pathway in cancer. Clin" 14 : 6735-6741, 2008

7 Buchholz, K. R., "The extracellular signal- regulated kinase/mitogen-activated protein kinase pathway induces the inflammatory factor interleukin-8 following Chlamydia trachomatis infection" 75 : 5924-5929, 2007

8 Buchholz, K. R., "The cytosolic pattern recognition receptor NOD1 induces inflammatory interleukin-8 during Chlamydia trachomatis infection" 76 : 3150-3155, 2008

9 De Kimpe, S. J., "The cell wall components peptidoglycan and lipoteichoicacid from Staphylococcus aureus act in synergy to cause shockand multiple organ failure" 92 : 10359-10363, 1995

10 Ohshima, Y., "Stimulation of human monocyte chemiluminescence bystaphylococcal lipoteichoic acid" 177 : 115-121, 1988

11 Rainard, P., "Staphylococcus aureus lipoteichoic acid triggers inflammation in the lactating bovine mammary gland" 39 : 52-, 2008

12 O’Hanlon, D. M., "Soluble adhesion molecules (E-selectin, ICAM-1 and VCAM-1) in breast carcinoma" 38 : 2252-2257, 2002

13 Ginsburg, I, "Role of lipoteichoic acid in infection and inflammation" 2 : 171-179, 2002

14 Rosette, C., "Role of ICAM1 in invasion of human breast cancer cells" 26 : 943-950, 2005

15 Chan, K. G., "Role of D-alanylation of Streptococcus gordonii lipoteichoic acid in innate and adaptive immunity" 75 : 3033-3042, 2007

16 Fernandes, A. F., "Proteasome inactivation promotes p38 mitogen-activated protein kinase-dependent phosphatidylinositol 3-kinase activation and increases interleukin-8 production in retinal pigment epithelial cells" 16 : 3690-3699, 2009

17 Mattsson, E., "Peptidoglycan and teichoic acid from Staphylococcus epidermidis stimulate human monocytes to release tumour necrosis factor-alpha, interleukin-1β and interleukin-6" 7 : 281-287, 1993

18 Erdman, S. E., "Nitric oxide and TNF-alpha trigger colonic inflammation and carcinogenesis in Helicobacter hepaticus-infected, Rag2-deficient mice" 106 : 1027-1032, 2009

19 Naugler, W. E., "NF-kappaB and canceridentifying targets and mechanisms" 28 : 19-26, 2008

20 Bougarn, S., "Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells" 17 : 1797-1809, 2010

21 Jang, K. S., "Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum" ACADEMIC PRESS INC ELSEVIER SCIENCE 407 (407): 823-830, 2011

22 Modi, W. S., "Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily" 84 : 185-187, 1990

23 Otte, J. M., "Mechanisms of cross hyporesponsiveness to Toll-like receptor bacterial ligands in intestinal epithelial cells" 126 : 1054-1070, 2004

24 Huang, P., "MAPK signaling in inflammation- associated cancer development" 1 : 218-226, 2010

25 Knapp, S., "Lipoteichoic acidinduced lung inflammation depends on TLR2 and the concerted action of TLR4 and the platelet-activating factor receptor" 180 : 3478-3484, 2008

26 Meron-Sudai, S., "Lipoteichoic acid synergizes with glycosphingolipids to potently stimulate secretion of interleukin-6 from human blood cells" 15 : 309-315, 2008

27 Kim, H. G., "Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharideinduced TNF-alpha production in THP-1 cells and endotoxin shock in mice" 180 : 2553-2561, 2008

28 서호성, "Lipoteichoic acid is important in innate immune responses to gram-positive bacteria" AMER SOC MICROBIOLOGY 76 (76): 206-213, 200801

29 Standiford, T. J., "Lipoteichoic acid induces secretion of interleukin-8 from human blood monocytes: a cellular and molecular analysis" 62 : 119-125, 1994

30 Kim, H. G., "Lipoteichoic acid from Lactobacillus plantarum elicits both the production of interleukin-23p19 and suppression of pathogen-mediated interleukin-10 in THP-1 cells" BLACKWELL PUBLISHING 49 : 205-214, 2007

31 Triantafilou, M., "Lipoteichoic acid and tolllike receptor 2 internalization and targeting to the Golgi are lipid raft-dependent" 279 : 40882-40889, 2004

32 Kim, H. J., "Lipoteichoic acid and muramyl dipeptide synergistically induce maturation of human dendritic cells and concurrent expression of proinflammatory cytokines" 81 : 983-989, 2007

33 Kolios, G., "Interleukin-8 production by the human colon epithelial cell line HT-29: modulation by interleukin- 13" 119 : 351-359, 1996

34 Kim, Han Geun, "Inhibitory Effects of Lactobacillus plantarum Lipoteichoic Acid (LTA) onStaphylococcus aureus LTA-Induced Tumor Necrosis Factor-Alpha Production" 한국미생물·생명공학회 18 (18): 1191-1196, 2008

35 Dinesh Thapa, "Inhibitory Effects of Clotrimazole on TNF-α-induced Adhesion Molecule Expression and Angiogenesis" 대한약학회 32 (32): 593-603, 2009

36 Danese, S., "Inflammatory bowel disease and intestinal cancer : a paradigm of the Yin-Yang interplay between inflammation and cancer" 29 : 3313-3323, 2010

37 Terzic, J., "Inflammation and colon cancer" 138 : 2101-2114, 2010

38 Ellingsen, E. A., "Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14" 8 : 149-156, 2002

39 Ellingsen, E., "Induction of cytokine production in human T cells and monocytes by highly purified lipoteichoic acid: involvement of Toll-like receptors and CD14" 8 : 149-156, 2002

40 Kolios, G., "Inducible nitric oxide synthase activity and expression in a human colonic epithelial cell line, HT-29" 116 : 2866-2872, 1995

41 양지현, "Impaired osteoclastogenesis by staphylococcal lipoteichoic acid through Toll-like receptor 2 with partial involvement of MyD88" FEDERATION AMER SOC EXP BIOL 86 : 823-831, 200910

42 Son, Y. M., "Immunomodulatory effect of resistin in human dendritic cells stimulated with lipoteichoic acid from Staphylococcus aureus" ACADEMIC PRESS INC ELSEVIER SCIENCE 376 (376): 599-604, 2008

43 Jong Suk Lee, "Grifola frondosa water extract alleviates intestinal inflammation by suppressing TNF-α production and its signaling" 생화학분자생물학회 42 (42): 143-154, 2010

44 Ryu, Y. H., "Differential immunostimulatory effects of Gram-positive bacteria due to their lipoteichoic acids" ELSEVIER SCIENCE BV 9 : 127-133, 2009

45 Mantovani, A., "Cancer-related inflammation" 454 : 436-444, 2008

46 Singh, S., "CXCL8 and its cognate receptors in melanoma progressionand metastasis" 6 : 111-116, 2010

47 Browning, D. D., "Autocrine regulation of interleukin-8 production in human monocytes" 279 : 1129-1136, 2000