Cytotoxic and Antimicrobial Activities of Cantharellus cibarius Fr. (Cantarellaceae)

다국어 초록 (Multilingual Abstract)

Antibacterial and cytotoxic activities of cyclohexane, dichloromethane, methanol, and aqueous extracts of Cantharellus cibarius were tested. Broth microdilution assay was performed against 10 bacterial strains (Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus subtilis, Enterococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella abony), with emphasis on Helicobacter pylori. Methanol extract was the most active against H. pylori strains with minimal inhibitory concentration values between 4 and 32 μg/mL. All extracts were active against antibiotic resistant H. pylori. Methanol and aqueous extracts had no cytotoxicity against tested cell lines, whereas cyclohexane and dichloromethane extracts were active against HeLa and N87 cells, but also against healthy MRC-5 cells (IC50 39.26 ± 1.24–134.79 ± 0.01 μg/mL). The tested aqueous extracts have shown 68% of angiotensin-converting enzyme inhibitory activity in doses of 1.25 mg/mL. Chemical analysis has shown the presence of linoleic, cis-vaccenic, and oleic acids, sterols, β-glucans, and polyphenolic compounds.

참고문헌 (Reference)

1 Barros L, "Wild and commercial mushrooms as source of nutrients and nutraceuticals" 46 : 2742-2747, 2008

2 Fons F, "Volatile compounds in the Cantharellus, Craterellus and Hydnum genera. Cryptogamie" 24 : 367-376, 2003

3 Kundakovic´ T, "Therapeutic properties of mushrooms in managing adverse effects in the metabolic syndrome" 13 : 2734-2744, 2013

4 정성우, "The antibacterial effect of fatty acids on Helicobacter pylori infection" 대한내과학회 31 (31): 30-35, 2016

5 Villares A, "Storage temperature and UV-irradiation influence on the ergosterol content in edible mushrooms" 147 : 252-256, 2014

6 Villares A, "Separation and characterization of the structural features of macromolecular carbohydrates from wild edible mushrooms" 2 : 15-21, 2013

7 Costa-Silva F, "Selenium contents of Portuguese commercial and wild edible mushrooms" 126 : 91-96, 2011

8 Tinggi U, "Selenium : Its role as antioxidant in human health" 3 : 102-108, 2008

9 Ohno M, "Rapid colorimetric assays for the quantification of leukemia inhibitory factor(LIF)and interleukin-6(IL-6)" 145 : 199-203, 1991

10 Mosmann T, "Rapid colorimetric assay for cellular growth and survival : Application to proliferation and cytotoxicity assays" 65 : 55-63, 1983

11 Clinical and Laboratory Standards Institute (CLSI), "Performance standards for antimicrobial Susceptibility testing: 24th Informational Supplement" NAT'L COMM CLINICAL LAB STANDARDS 2014

12 Skubatz H, "Oleic acid and its positional isomer, cis-vaccenic acid, in the appendix of Sauromatum guttatum during anthesis" 107 : 1433-1438, 1995

13 Association of Official Analytical Chemists(AOAC), "Official methods of analysis" Association of Official Analytical Chemists Inc 1990

14 Kozarski M, "Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius(Fries) : Primary mechanisms" 6 : 1875-1886, 2015

15 최춘환, "Multidrug Resistance Reversal Activity of Methanol Extracts from Basidiomycete Mushrooms in Cancer Cells" 한국생약학회 18 (18): 239-243, 2012

16 Falandysz J, "Macro and trace mineral constituents and radionuclides in mushrooms : Health benefits and risks" 97 : 477-501, 2013

17 Falandysz J, "Macro and trace elements in Common Chanterelle(Cantharellus cibarius)mushroom from the European background areas in Poland : Composition, accumulation, dietary exposure and data review for species, J Env Sci Health—Part B Pesticides" 50 : 374-387, 2015

18 Vetter J, "Lithium content of some common edible wild-growing mushrooms" 90 : 31-37, 2005

19 Kuipers EJ, "Helicobacter pylori and the risk and management of associated diseases : Gastritis, ulcer disease, atrophic gastritis and gastric cancer" 11 : 71-88, 1997

20 Ayala G, "Exploring alternative treatments for Helicobacter pylori infection" 20 : 1450-1469, 2014

21 Petrovic´ J, "Different extraction methodologies and their influence on the bioactivity of the wild edible mushroom Laetiporus sulphureus(Bull. )Murrill" 5 : 2948-2960, 2014

22 Prosky L, "Determination of insoluble and soluble dietary fiber in foods and food products : Collaborative study" 75 : 360-367, 1992

23 Kundakovic´ T, "Cytotoxic, antioxidant and antimicrobial activities of Ampelopsis brevipedunculata and Parthenocissus tricuspidata" 60 : 641-647, 2008

24 Jurca SJ, "Common substances that may contribute to resistant hypertension, and recommendations for limiting their clinical effects" 18 : 73-, 2016

25 David F, "Column Selection for the Analysis of Fatty Acid Methyl Esters"

26 Vamanu E, "Bioactive compounds, antioxidant and antiinflammatory activities of extracts from Cantharellus cibarius" 65 : 372-379, 2014

27 Mazurkiewicz N, "Bioaccumulation of trace elements in wild-growing edible mushrooms from Lubuskie voivodeship. Poland" 30 : 110-117, 2014

28 Palacios I, "Antioxidant properties of phenolic compounds occurring in edible mushrooms" 128 : 674-678, 2011

29 Kosanic M, "Antioxidant and antimicrobial properties of mushrooms" 19 : 1040-1046, 2013

30 Maria Makropoulou, "Antioxidant and Cytotoxic Activity of the Wild Edible Mushroom Gomphus clavatus" 한국식품영양과학회 15 (15): 216-221, 2012

31 Sisto F, "Antimicrobial susceptibility testing of Helicobacter pylori determined by microdilution method using a new medium" 58 : 559-563, 2009

32 Kolund zic´ M, "Antibacterial and cytotoxic activities of wild mushroom Fomes fomentarius(L. )Fr., Polyporaceae. Ind" 79 : 110-115, 2016

33 Seniuk OF, "Anti-infective properties of the melanin-glucan complex obtained from medicinal tinder bracket mushroom, Fomes fomentarius(L. : Fr. )Fr. (Aphyllophoromycetideae)" 13 : 7-18, 2011

34 Muszyn´ska B, "Analysis of indole compounds in edible Basidiomycota species after thermal processing" 132 : 455-459, 2012

35 Wang XM, "A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China" 151 : 279-285, 2014