Inhibition of Basic Fibroblast Growth Factor Induced Corneal Angiogenesis by a Farnesyl Transferase Inhibitor

다국어 초록 (Multilingual Abstract)

Farnesyl transferase inhibitors (FTI) disrupt fanesylation of ras protein and thus, suppress tumor growth in vivo, To determine whether FTI extracted from Cinnamomum Cassia Blume (CB2'-ph) interferes with angiogenesis, we studied the effect of CB2'-ph on rabbit corneal angiogenesis induced by basic fibroblast growth factor (bFGF).
A hydrogel disk containing 1000ng of bFGF was implanted intrastromally in the superior cornea of 12 NZW rabbit eyes. All eyes received a second intrastromal disk, randomized to contain either 40㎍ of CB2'-ph(n=6) or phosphate-buffered saline (PBS) (n=6). Both disks were positioned side-by-side, 1.2mm from the superior limbus. Each eye was examined daily by two masked of new blood vessels.
At 3, 5, and 7 days postimplantation of bFGF disks, eyes treated with CB2'-ph showed mean angiogenesis score of 6.0 ± 4.8, 25.6 ± 23.9 and 38.1 ± 28.3, respectively, while PBS-treated controls scored 10.4 ± 9.2, 27.2 ± 16.7, and 39.0 ± 22.8, respectively (p＞0.4, Wilcoxon signed rank test).
In a rabbit corneal pocket assay, CB2'-ph appears to be ineffective against bFGF-induced corneal angiogenesis in the model. (Journal of Korean Ophthalmology Society 40:657-661, 1999)