The purpose of the study was to evaluate the impact of treadmill endurance exercise(TEE) and/or Sildenafil(SIL) treatment, a PDE5 inhibitor on pathological main hallmarks and cognitive deficits of AlCl3-induced Alzheimer's Disease(AD) mice model. To i...
The purpose of the study was to evaluate the impact of treadmill endurance exercise(TEE) and/or Sildenafil(SIL) treatment, a PDE5 inhibitor on pathological main hallmarks and cognitive deficits of AlCl3-induced Alzheimer's Disease(AD) mice model. To induce AD in an animal model, all mice except the CON group were administrated AlCl3 (40mg/kg) for 60 days. After last treatment, AlCl3-induced AD mice were conducted TEE(10~20m/min, 50 min, 5 days/week) and/or SIL treatment(7mg/kg/day, 5 days/week) for 4 weeks.
Increased the level of amyloid-β and cleaved caspase-3 induced by AlCl3 was decreased following TEE and/or SIL treatment. In addition, low-density lipoprotein receptor-related protein-1(LRP-1) protein was reduced and the receptor for advanced glycation end products (RAGE) was increased induced by AlCl3 treatment. However, only LRP-1 protein was elevated following TEE and/or SIL treatment. Moreover, reduced antioxidant enzymes such as superoxide dismutase-1(SOD-1), superoxide dismutase-2(SOD-2), and catalase were increased by TEE and/or SIL treatment. Finally, cognitive function and Nest formation score were significantly reduced following AlCl3 treatment, but it was improved following TEE and/or SIL treatment. Collectively, our data suggested that TEE and/or SIL treatment may regulate amyloid-β expression, cerebrovascular function, and antioxidant enzymes, thereby ameliorating AlCl3-induced cognitive deficits in AD mice.