Tomato plants were regenerated from callus-derived protoplasts. Plants regeneration depended on the genotypes and the kinds of callus growth media. A good shoot formation was obtained from the callus of PI 1999380 grown in a 2,4-D mediu, added. Also t...
Tomato plants were regenerated from callus-derived protoplasts. Plants regeneration depended on the genotypes and the kinds of callus growth media. A good shoot formation was obtained from the callus of PI 1999380 grown in a 2,4-D mediu, added. Also the best shoot formation was obtained with 2.0㎎/l zeatin alone. The M-MS medium supplemented with 0.5㎎/l BAP and 1.0㎎/l NAA increased plating efficiency of potato protoplasts. Hybrid-microcolony was obtained from the fused protoplasts. Tomato protoplasts co-cultivated with potato leaf DNA proliferated to calli.