A Chloroplast-targeted S1 RNA-binding Domain Protein Plays a Role in Arabidopsis Response to Diverse Abiotic Stresses

다국어 초록 (Multilingual Abstract)

Although accumulating evidence points to theessential roles of nucleus-encoded chloroplast S1 domaincontainingproteins (SDPs) in chloroplast RNA metabolismand plant development, functions of chloroplast SDPs inabiotic stress responses are largely unknown. In this study,we investigated the role of a SDP (At1g12800) in Arabidopsisresponse to diverse abiotic stresses. Analysis of the sdpknockout mutant and complementation lines demonstratedthat loss of SDP function results in decreased survival rate ofArabidopsis under salt, heat, UV, or freezing stress, but notunder dehydration stress or ABA. In consistent with aprevious report demonstrating that SDP is involved inchloroplast rRNA processing, translation in chloroplasts wasimpaired in the sdp mutant. Expression of several nucleargenes involved in stress response and adaptation was alteredin the sdp mutant subjected to different abiotic stresses,suggesting that modulation of chloroplast translation affectsthe expression of nuclear genes under abiotic stresses. Thesedata reveal that chloroplast-localized SDP plays an importantrole in abiotic stress response by modulating chloroplasttranslation and the expression of nuclear genes possibly viaunidentified plastid-to-nucleus signaling.

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