Glufosinate-ammonium 저항성 형질전환벼의 저항성 수준과 적응성에 관한 연구 = Levels of Resistance and Fitness in Glufosinate-ammonium-Resistant Transgenic Rice Plants

다국어 초록 (Multilingual Abstract)

The objectives of this research were to quantify resistance levels of transgenic rice expressing the bar gene to glutamine synthetase (GS)-inhibiting, and methionine sulfoximine and photosynthesis-inhibiting herbicide, paraquat, and compare the ammonium accumulation, chilling injury, and yield between transgenic and non-transgenic rice. The transgenic rice lines were 45-96-fold more resistant to glufosinate-ammonium than non-transgenic rice. The transgenic rice lines were also 18-fold more resistant to methionine sulfoximine, but was not resistant to paraquat, which has different target site. Glufosinate-ammonium increased the ammonium accumulation in leaves of non-transgenic rice plants, but had minimal or no effect on leaves of transgenic lines. The transgenic lines except for 258, 411, 607 and 608 were more susceptible during chilling and recovery than non-transgenic rice plants. The yield of transgenic lines 142, 144, 258 and 608 was similar or higher than that of non-transgenic rice in pot conditions.

국문 초록 (Abstract)

본 연구의 목적은 bar 유전자를 발현한 형질전환벼에대한 글루타민 합성효소 저해제인 glufosinate-ammonium,L-metionine sulfoximine과 광합성저해제인 paraquat에 저항성 수준과 형질전환벼와 비형질전환벼의 암모늄 축적량,저온반응 및 수량을 비교하는데 있다. 형질전환벼 계통은비형질전환벼에 비해 glufosinate-ammonium에 45~95배 높은 저항성을 보였다. 한편 형질전환벼 계통은 L-metionine sulfoximine을 처리했을 때도 형질전환벼 계통이 비형질전환벼에 비해 18배 이상의 저항성을 보여 교차저항성이 있음을 확인할 수 있었다. 그러나 glufosinate-ammonium과작용기작이 다른 제초제인 paraquat에 대한 다중저항성은없었다. Glufosinate-ammonium 처리에 의해 ammonium 축적은 비형질전환벼에서 증가하였으나 모든 형질전환벼의계통에서는 적거나 효과가 없었다. 형질전환벼 계통 258,411, 607 및 608을 제외한 계통은 비형질전환벼에 비해저온 내성 및 저온 후 회복정도가 유의적으로 낮은 경향을 보였다. 형질전환벼 계통 142, 144, 258 및 608의 수량은 비형질전환벼와 유사하거나 높았다.

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