TRAF6 is a key immune gene that plays a significant role in toll-like receptor signal transduction andactivates downstream immune genes involved in antiviral immunity in fish. To explore the role ofTRAF6 in Epithelioma papulosum cyprini (EPC) cells, w...
TRAF6 is a key immune gene that plays a significant role in toll-like receptor signal transduction andactivates downstream immune genes involved in antiviral immunity in fish. To explore the role ofTRAF6 in Epithelioma papulosum cyprini (EPC) cells, we knocked out the TRAF6 gene using theClustered Regularly Interspaced Short Palindromic Repeats-Cas9 (CRISPR-Cas9) technique andthen analyzed the transcriptomes of the knockout cells. In this study, we identified that 232transcripts were differentially expressed in naive cells. Using the pipeline, we identified 381novel lncRNAs in EPC cells, 23 of which were differentially expressed. Gene Ontologyenrichment analysis demonstrated that differentially expressed genes (DEG) are implicated invarious immune processes, such as neutrophil chemotaxis and mitogen-activated protein kinasebinding. In addition, the KEGG pathway analysis revealed enrichment in immune-relatedpathways (Interleukin-17 signaling pathway, cytokine-cytokine receptor interaction, and TNFsignaling pathway). Furthermore, the target genes of the differentially expressed lncRNAs wereimplicated in the negative regulation of interleukin-6 and tumor necrosis factor production.
These results indicate that lncRNAs and protein-coding genes participate in the regulation ofimmune and metabolic processes in fish.