Genetic Diversity and Inter-relationships among MaizeInbred Lines using MITE-AFLP

다국어 초록 (Multilingual Abstract)

MITEs (miniature inverted-repeat transposable elements) are one of the major transposable elements in maize. We haveapplied MITE-AFLP technique to study the genetic diversity and genetic relationship among 25 elite maize inbred lines. WhileMITE-AFLP fragment sizes ranged approximately from 100 to 650 bp, the fragments in the range of 200-400 bp showed higherproportion of polymorphic bands than monomorphic bands. Analysis of the 25 inbred lines with 8 MITE-AFLP primer combi-nations detected a total of 229, of which 130 (57.4%) were polymorphic among the inbreds. The 25 inbred lines utilized consistedof mainly two groups of Lancaster Sure Crops in BSSS, Reid Yelow Dent, and one miscellaneous line. The genetic distances,GDs, between groups were slightly higher than the GDs within the groups. Shannon's index of diversity Hs of MITE-AFLP vari-ations for LSC and BS inbred were 4.55 and 4.9, respectively. Thus, the BSSS inbred lines exhibited relatively higher variationthan LSC inbred lines. In the cluster analysis of 25 inbred lines, four major groups were recognized; Group I contained 4 linesfrom LSC and 1 line from BSSS, Group I contained 6 lines from BSSS, 2 lines from LSC and ND203 from diferent origin,Group II contained 3 lines from BSSS and one line from LSC, Group IV contained 5 lines from BSSS and 2 lines from LSC.Since the clustering of the maize inbreds based on MITE-AFLP matched with the lineages except of a few odd lines, the MITE-AFLP analysis might provide a new insight in studies of genetic diversity and select for beter combination for hybrid breeding inmaize.