The Autophagy Protein CsATG8 is Involved in Asexual Development and Virulence in the Pepper Anthracnose Fungus Colletotrichum scovillei

다국어 초록 (Multilingual Abstract)

Autophagy serves as a survival mechanism and plays important role in nutrient recycling under conditions of starvation, nutrient storage, ad differentiation of plant pathogenic fungi.
However, autophagy-related genes have not been investigated in Colletotrichum scovillei,a causal agent of pepper fruit anthracnose disease. ATG8 is involved in autophagosome forma- tion and is considered a marker of autophagy. Therefore, we generated an ATG8 deletion mutant, DCsatg8, via homologous recombination to determine the functional roles of CsATG8 in the development and virulence of C. scovillei. Compared with the wild-type, the deletion mutant DCsatg8 exhibited a severe reduction in conidiation. Conidia produced by DCsatg8 were defective in survival, conidial germination, and appressorium formation.
Moreover, conidia of DCsatg8 showed reduced lipid amount and PTS1 selectivity. A virulence assay showed that anthracnose development on pepper fruits was reduced in DCsatg8.
Taken together, our results suggest that CsATG8 plays various roles in conidium production and associated development, and virulence in C. scovillei.

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