桂枝芍藥知母湯이 DNCB로 유발된 알레르기성 접촉피부염에 미치는 영향 = (The) effects of Gyejijakyakjimo-tang on the allergic contact dermatitis induced by DNCB

다국어 초록 (Multilingual Abstract)

Background and Objectives : It could be said industrialization gave rise to the disease Allergic Contact Dermatitis. As the industrialization advances, the more materials that could provake the allergic contact dermatitis were increasing. Therefore, in oriental medicine, various studies have been performed. The purpose of this study is to investigate the effects of Gyejijakyakjimo-Tang on the Allergic Contact Dermatitis caused by 2,4-dinitro-chlorobezene(DNCB).
Meterial and Methods : Twenty eight mice were divided into four groups ; normal, control, experimental group A and B. Control and experimental groups were induced allergic contact dermatitis by DNCB. Experimental group A was orally administered the Gyejijakyakjimo-Tang and experimental group B was orally administered the prednisolone. In this study, ear thickness measurement, auricle microphotograph observation, MPO(Myeloperoxidase) activity measurement, Reverse transcription-polymerase chain reaction(RT-PCR) analysis of the mRNA level of TNF-α, IL-1β were performed on these four groups. In addition, the effect of Gyejijakyakjimo-Tang on cell viability and the effect of Gyejijakyakjimo-Tang on the compound 48/80-induced histamine release from HMC and RPMC were measured.

Results :
1. Both experimental group A and B had decreased ear thickness compared with control group In contact hypersensitivity assay.
2. In experimental group A, inflammatory edema was similarly observed comparing to control group. Nevertheless, inflammatory edema was obviously reduced in experimental group B. In both experimental group A and B, pathological lesion of dermatitis were alleviated. In addition, the numbers of infiltrated inflammatory cells were decreased compared with control group.
3. Compared to the normal group, there was a noticeable increase in MPO activity in control group. However, in experimental group A and B, it showed remarkable inhibition of the increase in MPO activity comparing with control group.
4. The level of expression of TNF-α, IL-1β in experimental group A and B were meaningfully lower than those in control group.
5. In MTT assay, the concentrations of Gyejijakyakjimo-Tang that were used on the test had no cytotoxicity.
6. Gyejijakyakjimo-Tang dose-dependently inhibited the compound 48/80-induced histamine release from both HMC and RPMC.

Conclusions : According to above experiments, Gyejijakyakjimo-Tang was effective on allergic contact dermatitis.

목차 (Table of Contents)

• Ⅰ. 서 론 1
• Ⅱ. 실 험 방 법 3
• 1. 실험동물, 세포배양 및 재료 3
• 1) 실험동물 3
• 2) 세포배양 3
• 3) 처방 구성 및 시료 제조 3
• 2. 실험방법 4
• 1) 실험군 4
• 2) 알레르기성 접촉피부염 병태 모델 5
• 3) 시료의 투여 5
• 4) 귀의 부종 측정 5
• 5) 귀의 조직 관찰 5
• 6) Reverse transcription-polymerase chain reaction(RT-PCR) 6
• 7) Myeloperoxidase(MPO) activity 7
• 8) MTT assay 7
• 9) 사람 비만세포(HMC)에서 histamine 유리량 측정 7
• 10) 흰쥐 복강 비만세포(RPMC)에서 histamine 유리량 측정 8
• 11) 통계처리 8
• Ⅲ. 실 험 성 적 10
• 1. 귀의 부종 변화에 미치는 영향 10
• 2. 조직학적 관찰 12
• 3. 호중구의 침착에 미치는 영향 15
• 4. Proinflammatory cytokine의 유전자 발현에 미치는 영향 16
• 5. 세포 독성 평가 17
• 6. 사람 비만세포와 흰쥐 복강 비만세포에서 histamine의 유리량에
• 미치는 효과 18
• Ⅳ. 고 찰 21
• Ⅴ. 결 론 28
• 참 고 문 헌 29
• Abstracts 33