Cellular toxicity driven by high-dose vitamin C on normal and cancer stem cells

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>As a powerful antioxidant, vitamin C protects cells from oxidative damage by inhibiting production of free radicals. However, high levels of vitamin C shows cytotoxicity especially on cancerous cells through generating excessive ROS and blocking the energy homeostasis. Although the double-sided character of vitamin C has been extensively studied in many cell types, there is little research on the consequence of vitamin C treatment in stem cells. Here, we identified that high-dose vitamin C shows cellular toxicity on proliferating NSPCs. We also demonstrated that undifferentiated NSPCs are more sensitive to vitamin C-driven DNA damage than differentiated cells, due to higher expression of Glut genes. Finally, we showed that high-dose vitamin C selectively induces DNA damage on cancer stem cells rather than differentiated tumor cells, raising a possibility that vitamin C may be used to target cancer stem cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> High-dose vitamin C induces DNA damage and apoptosis on NSPCs. </LI> <LI> Undifferentiated NSPCs are more sensitive to vitamin C-driven DNA damage than differentiated cells. </LI> <LI> Undifferentiated NSPCs show higher expression of Glut genes than differentiated cells. </LI> <LI> High-dose vitamin C selectively induces DNA damage on cancer stem cells. </LI> </UL> </P>