<P><B>Abstract</B></P><P>The expression of <I>CBF</I> (C-repeat-binding factor) genes is required for freezing tolerance in <I>Arabidopsis thaliana. CBFs</I> are positively regulated by INDUCER OF ...
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https://www.riss.kr/link?id=A107434265
2017
-
SCI,SCIE,SCOPUS
학술저널
6613-6627(15쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P><B>Abstract</B></P><P>The expression of <I>CBF</I> (C-repeat-binding factor) genes is required for freezing tolerance in <I>Arabidopsis thaliana. CBFs</I> are positively regulated by INDUCER OF ...
<P><B>Abstract</B></P><P>The expression of <I>CBF</I> (C-repeat-binding factor) genes is required for freezing tolerance in <I>Arabidopsis thaliana. CBFs</I> are positively regulated by INDUCER OF CBF EXPRESSION1 (ICE1) and negatively regulated by MYB15. These transcription factors directly interact with specific elements in the <I>CBF</I> promoters. Mitogen-activated protein kinase (MAPK/MPK) cascades function upstream to regulate <I>CBFs</I>. However, the mechanism by which MPKs control <I>CBF</I> expression during cold stress signaling remains unknown. This study showed that the activity of MYB15, a transcriptional repressor of cold signaling, is regulated by MPK6-mediated phosphorylation. MYB15 specifically interacts with MPK6, and MPK6 phosphorylates MYB15 on Ser168. MPK6-induced phosphorylation reduced the affinity of MYB15 binding to the <I>CBF3</I> promoter and mutation of its phosphorylation site (MYB15<SUP>S168A</SUP>) enhanced the transcriptional repression of <I>CBF3</I> by MYB15. Furthermore, transgenic plants overexpressing MYB15<SUP>S168A</SUP> showed significantly reduced <I>CBF</I> transcript levels in response to cold stress, compared with plants overexpressing MYB15. The <I>MYB15<SUP>S168A</SUP></I>-overexpressing plants were also more sensitive to freezing than <I>MYB15</I>-overexpressing plants. These results suggest that MPK6-mediated regulation of MYB15 plays an important role in cold stress signaling in <I>Arabidopsis</I>.</P>