골격근의 신장성 및 단축성 근수축 후에 면역적격세포수의 변화 = Changes in Immunocompetent Cell Numbers Following Eccentric and Concentric Muscle Contraction in Skeletal Muscle

국문 초록 (Abstract)

본 연구는 골격근의 신장성 및 단축성 근수축 후에 면역적격세포수의 동태를 검증하는데 그 목적을 두었다. 실험동물은 체중 30-40g(8주령)의 ICR계 수컷 생쥐 총 84마리를 신장성 근수축 집단(ECC, n=42)과 단축성 근수축 집단(CON, n=42)으로 분류하였다. 신장성 및 단축성 근수축(30회 5세트, 각 세트간의 휴식 1분)은 전경골근의 비골신경을 통해 전기 자극을 주었다. 전경골근의 적출은 신장성 및 단축성 근수축 전(pre, n=6), 직후(IA, n=6), 1일 후(1D, n=6), 3일 후(3D, n=6), 7일 후(7D, n=6), 14일 후(14D, n=6), 그리고 28일 후(28D, n=6)에 각각 실시하였다. 양성 근섬유수 및 면역적격세포수(T, Th, Tc, 호중구, 포식세포)는 조직학적 분석을 통해 산출하였다. 양성 섬유수는 근수축 3일 및 7일 후에 CON 집단에 비해 ECC 집단에 있어서 유의하게 높게 나타났다(p<.05). Tc 세포수는 근수축 1일, 3일 및 7일 후에, 호중 구수는 근수축 직후, 1일, 3일 및 7일 후에, 포식 세포수는 근 수축 직후, 1일, 3일, 7일, 14일 및 28일 후에 각각 CON 집단에 비해 ECC 집단에 있어서 유의하게 높게 나타났다(p<.05). 반면, T 및 Th 세포수는 시기 및 집단 간에서 유의한 차이를 보이지 않았다. 이상의 결과에 의하면 신장성 근수축은 골격근 손상 및 면역적격세포수를 크게 증가시켰다는 것이 시사되었다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to examine immunocompetent cell numbers following eccentric and concentric muscle contraction in skeletal muscle. Eight-week-old Institute of Cancer Research (ICR) mice (n=84) were divided to eccentric muscle contraction group (ECC, n=42) and concentric muscle contraction group (CON, n=42). The tibialis anterior muscle was stimulated via the peroneal nerve to contract either eccentrically or concentrically (30 times × 5sets, Rest for 1 minute between each set). The tibialis anterior muscle was isolated before (pre, n=6) and 1 (n=6), 3 (n=6), 7 (n=6), 14 (n=6), and 28 d (n=6) after muscle contraction. Changes in the number of positive muscle fiber and immunocompetent cells (T, Th, Tc, neutrophils, and macrophages) were calculated by the histological analysis with HE stain and Immunohistochemistry. The number of positive muscle fiber was significantly higher (p<.05) in the ECC than in the CON at 3 and 7 d after muscle contraction. The number of Tc cells was significantly higher (p<.05) in the ECC than in the CON at 1, 3, and 7 d after the contractions. The number of neutrophils was significantly higher (p<.05) in the ECC than in the CON at immediately after, 1, 3, and 7 d after the contractions. The number of macrophages was significantly higher (p<.05) in the ECC than in the CON at all time course after muscle contraction. However, Changes in the number of T and Th cells were not significantly different between time course or groups. These results suggest that eccentric muscle contraction induces greater skeletal muscle damage and immunocompetent cells.

참고문헌 (Reference)

1 山田　茂, "腱切除法による骨格筋肥大時のマクロファージの役割" 48 : 509-514, 1999

2 Nehlsen-cannarella, S. L., "The effect of moderate exercise training on immune response" 23 : 64-70, 1991

3 Ferguson, A., "The effect of antigen deprivation on thymus-dependent and thymus-independent lymphocyte in the small intense of the mouse" 12 : 477-488, 1972

4 Abo, T., "The appearance of T cells receptor in the livers of mice injected with bacteria" 174 : 417-424, 1991

5 Mehrzad, J., "T lymphocyte proliferative capacity and CD4+/CD8+ ratio in primiparous and pluriparous lactation cows" 75 (75): 457-465, 2008

6 Friden, J., "Structural mechanical basis of exercise-induced muscle injury" 24 (24): 521-530, 1992

7 Belcastro, A., "Skeletal muscle calcium-activated neutral protease (calpain) with exercise" 74 (74): 1381-1386, 1993

8 Rhind, S. G., "Shephard, R. J. (1996). Effects of moderate endurance exercise and training on in vitro lymphocyte proliferation, interleukin(IL-2) production, and IL-2 receptor expression" 74 : 348-360, 1996

9 Chazaud, B., "Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth" 163 (163): 1133-1143, 2003

10 McLennan, I. S., "Resident macrophages (ED2- and ED3- positive) do not phagocytese degenerating rat skeletal muscle fibers" 272 : 193-196, 1993

11 Mizuno, K., "Oligoclonal expansion of circulating and tissue-infiltrating CD8+ T cells with killer/effector phenotypes in juvenile dermatomyosaitis syndrome" 137 (137): 187-194, 2004

12 Lamas, O., "Obesity and immunocompetence" 56 (56): S42-S45, 2002

13 Lockhart, N. C., "Neutrophil accmulation following passive stretches contributes to adaptations that reduce contraction-induced skeletal muscle injury in mice" 104 (104): 1109-1115, 2008

14 Arahata, K., "Monoclonal antibody analysis of mononuclear cells in myopathies. Ⅰ: Quantitation of subsets according to diagnosis and sites of accumulation and demonstration and counts of muscle fibers invaded by T cells" 26 : 193-208, 1984

15 Merly, F., "Macrophages enhance muscle satellite cell proliferation and delay their differentiation" 22 : 724-732, 1999

16 Balnave, C. D., "Intracellular calcium and force in single mouse muscle fibres following repeated contractions with stretch" 488 : 25-36, 1995

17 Armstrong, R. B., "Initial events in exercise-induced musclar injury" 22 (22): 429-435, 1990

18 Tidball, J. G., "Inflammatory cell response to action muscle injury" 27 (27): 1022-1032, 1995

19 Malm, C., "Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies" 529 : 243-262, 2000

20 Shore, S., "Immune responses to training: how critical is training volume?" 39 : 1-11, 1999

21 Beauchamp, J. P., "Expression and function of heterotypic adhension molecules during differentiation of human skeletal muscle in culture" 140 : 387-410, 1992

22 Jones, D. A., "Experimental human muscle damage: morphological changes in relation to other indices of damage" 375 : 435-448, 1986

23 Pizza, F. X., "Exercise-induced muscle damage: effect on circulating leukocyte and lymphocyte subsets" 27 (27): 363-370, 1995

24 Peake, J. M., "Exercise- induced muscle damage, plasm cytokines, and markers of neutrophil activation" 37 : 737-745, 2005

25 Sorichter, S., "Effects of unaccustomed and accustomed exercise on the immune response in runners" 38 (38): 1739-1745, 2006

26 Pedersen, B. K., "Effects of exercise on lymphocytes and cytokines" 34 : 246-251, 2000

27 Kon, M., "Eccentric muscle contractions unduce greater oxidative stress than concentric contractions in skeletal muscle" 32 : 273-282, 2007

28 Lee, J., "Eccentric exercise effect on blood oxidative-stress markers and delayed onset of muscle soreness" 34 : 443-448, 2002

29 Stauber, W. T., "Eccentric action of muscle: physiology, injury, and adaptation" 17 : 157-185, 1988

30 Massimino, M. L., "ED2+ macrophages increase selectively myoblast proliferation in muscle cultures" 235 : 754-759, 1997

31 Altres, S. E., "Dendritic cells pulsed with CEA peptide induce CEA-specific CTL with restricted TCR repertoire" 21 : 17-26, 1998

32 Knight, K. L., "Cryotherapy in sports injury management" Sports medicine Quarterly 1997

33 Tvede, N., "Cellular immunity in highly trained elite racing cyclists during periods of training with high and low intensity" 1 : 163-166, 1991

34 Fielding, R. A., "Acute phase response in exercise Ⅲ. Neutrophil and IL-1 beta accumulation in skeletal muscle" 265 : R166-R172, 1993

35 Warren, G. L., "A stimulating nerve cuff for chronic in vivo measurements of torque produced about the ankle in the mouse" 84 (84): 2171-2176, 1998

36 이호성, "12개월간의 운동이 고령자의 림프구 아집단에 미치는 영향" 한국운동생리학회 15 (15): 173-180, 2006