cDNA CLONING OF A SEED-SPECIFIC GENE FROM SOYBEAN

다국어 초록 (Multilingual Abstract)

To improve the nutritional quality of soybean seed protein, overexpression of sulfur-rich protein was attempted. A cDNA clone SK41 was isolated from soybean seed cDNA library shows 48% similarity with the the probe and the content of sulfur-containing amino acids reaches about 12%. The features of deduced polypeptide are the presence of nine contiguous aspartic acids, putative cell-attatchment sequence (Arg-Gly-Asp) and two potential N-glycosylation sequences (Asn-Leu-Ser, Asn-Gln-Ser). A part deduced amino acid sequence of the clone SK41 shows 97.7% identity with aspartic acid-rich peptide of soybean. Northern blot analysis shows that the clone SK41 is expressed specifically in seeds with the maturation as other seed protein genes. The expression pattern at transcriptional level was much the same as major soybean seed storage protein, glycinin. The clone SK41 was expressed under the control of T7 promoter system in E. coli and purified by immobilized metal ion affinity chromatography (IMAC). Polyclonal antibody was raised against it, then immunoblot analysis was carried out. The protein of 36kD in young seeds and the protein of 22kD in mature seeds was recognized by antibody for SK41 protein. According to seed maturation, it seems that the protein of 36 kD is processed to the protein of 22 kD. The protein of 22kD shows the same expression pattern as glycinin, seed storage protein of which the amount increases with seed maturation.

목차 (Table of Contents)

• Ⅰ. Introduction = 175
• Ⅱ. Main Contents = 175
• Ⅲ. Summary = 180