Conversion of lysine into cadaverine by whole cell biocatalysts using Escherichia coli ldcC = Conversion of lysine into cadaverine by whole cell biocatalysts using Escherichia coli ldcC

다국어 초록 (Multilingual Abstract)

1,5-diaminopentane(cadaverine) is a valuable chemical because of its wide industrial applications. Since there are not many reports of the direct production using biocatalysts, the enzymatic process using lysine decarboxylases(LDCs) was developed for cadaverine production from renewable material such as lysine. There are two well-known LDCs in Escherichia coli, cadA and ldcC. Among them, cadA is more popular than ldcC because cadA has two times higher biological activity in comparison with ldcC. In this study, lysine was converted into cadaverine by whole cell biocatalysts using E.coli BL21(DE3) expressing E.coli ldcC. In order to overcome the low expression level and the low conversion rate of ldcC, the effects of IPTG concentration and conversion temperature and pH were studied to increase the expression of ldcC and the conversion rate of lysine into cadaverine. Higher expression level of ldcC was observed, and 95% of lysine was consumed for conversion into cadaverine.