Basic, HCCbasic : PO-21 ; Increased apolipoprotein E in hepatocellular carcinoma = Basic, HCCbasic : PO-21 ; Increased apolipoprotein E in hepatocellular carcinoma

다국어 초록 (Multilingual Abstract)

Background: Hepatocellular carcinoma (HCC) is one of the most malignant tumors worldwide. The goal of our study was to identify new biomarkers for HCC for early detection and for gaining an improved understanding of hepatocarcinogenesis by performing a comparative proteomic analysis of HCC and adjacent non-tumor tissue. Methods: Pairs of tumor and adjacent non-tumor tissue were obtained from 167 HCC patients after surgical resection at Ajou University Hospital, Korea. We assessed the histopathological features and additional clinical data. Proteomic analysis of tumor and non-tumor tissue revealing differential expression using 2D electrophoresis and MALDI-TOF MS analysis reveals some xenobiotic enzymes and apo E was selected. The associations between the expression of apoE and the clinical and pathological parameters were analyzed using SPSS. Results: One of the xenobiotic enzymes, apoE was significantly increased in tumor tissue compared with surrounding nontumor tissue. Paired T-test showed significant high level of apoE in tumor compared with non-tumor (209.91 vs 87.59, p < .01). Oneway ANOVA revealed that levels of apoE were elevated independently among different Child classes, etiologies, sex and consumption of alcohol. Conclusions: We compared the protein profiles between tumor and non-tumor tissue in HCC patients. This study revealed that apoE was significantly higher in HCC than in non-tumor tissue. The multivariate analysis confirmed apoE was higher in regardless of Child class, etiology, sex and alcohol consumption. ApoE has long been known as plasma lipoproteins responsible for cholesterol transport and metabolism. Plasma apoE is produced mainly in the liver but also in the brain, adrenal glands, kidney, and macrophage and not increased in gene expression and serum level in patients with HCC. Some proteomic studies reports a strong relationship between other cancers and apoE. We suppose that this report add a clue to understand hepatocarcinogenesis from hepatic steatosis to consequently hepatic carcinoma.