임플란트 주위 골 결손 부위에 Choukroun's Platelet-rich-fibrin와 실크 분말 복합 이식재 사용 = Effect of Combination Graft of Choukroun's Platelet-rich-fibrin with Silk Fibroin Powder in the Peri-implant Defects

다국어 초록 (Multilingual Abstract)

Purpose: Choukroun's platelet-rich-fibrin (PRF) is composed of platelets, white blood cells and fibrin matrix. It does not induce enough bone formation by itself but it can improve bone formation with calcium. Silk fibroin does not cause inflammatory reactions because it is bio-compatible and degradable. The purpose of this study was to exam the bone formation when a combination of Choukroun's PRF and silk fibroin was used. Methods: In this study, cell reactions to silk powder with differing molecular weights was first tested to select the appropriate silk powder. Then we applied these bone graft materials on defects of skull and in a peri-implant bony defect model in New Zealand rabbits. The results between the experimental and control s (non-grafted) group were analyzed. Results: The small sized silk fibroin powder showed increased cellular proliferation for bone-regeneration. There was no statistically significant difference between the experimental group and the control group at 6 weeks, but more new bone formation was observed in the combination graft group at 12 weeks (P<0.05). And in the dental implant model, the combination bone graft group showed much improved torque test results, which was statistically significant. Histomorphometric analysis showed more regenerated cortical bone and a higher mean bone to implant in the experimental group. Both were statistically significant. Conclusion: The combination graft of Choukroun's platelet-rich-fibrin (PRF) and silk fibroin powder can successfully restore the bony defects in a skull defected model and a peri-implant bony defects model.

참고문헌 (Reference)

1 Roh DH, "Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat" SPRINGER 17 : 547-552, 2006

2 Altman GH, "Silk-based biomaterials" 24 : 401-416, 2003

3 Williams CM, "Schwarzbauer JE. Fibronectin expression modulates mammary epithelial cell proliferation during acinar differentiation" 68 : 3185-3192, 2008

4 Huys LW, "Replacement therapy and the immediate post-extraction dental implant" 10 : 93-102, 2001

5 Cai K, "Poly(D, L-lactic acid) surfaces modified by silk fibroin: effects on the culture of osteoblast in vitro" 23 : 1153-1160, 2002

6 Ferreira CF, "Platelet-rich plasma influence on human osteoblasts growth" 16 : 456-460, 2005

7 Casati MZ, "Platelet-rich plasma does not improve bone regeneration around peri-implant bone defects-a pilot study in dogs" 36 : 132-136, 2007

8 Jung RE, "Platelet-rich plasma and fibrin as delivery system for rhBMP-2" 16 : 676-682, 2005

9 Dohan DM, "Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet- related biological features" 101 : e45-e50, 2006

10 Dohan DM, "Platelet- rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution" 101 : e37-e44, 2006

11 Cassinelli C, "Physical-chemical and biological characterization of silk fibroin-coated porous membranes for medical applications" 29 : 881-892, 2006

12 Hwang KG, "Partial-thickness cortical bone graft from the mandibular ramus: a non-invasive harvesting technique" 79 : 941-944, 2008

13 Sykaras N, "Osseointegration of dental implants complexed with rhBMP-2: a comparative histomorphometric and radiographic evaluation" 19 : 667-678, 2004

14 Mori H, "New silk protein: modification of silk protein by gene engineering for production of biomaterials" 74 : 95-103, 2000

15 Smith JC, "Mesoderm induction and the control of gastrulation in Xenopus laevis: the roles of fibronectin and integrins" 108 : 229-238, 1990

16 Verardi S, "Management of the exposure of e-PTFE membranes in guided bone regeneration" 19 : 111-117, 2007

17 Fiorellini JP, "Localized ridge augmentation/preservation. A systematic review" 8 : 321-327, 2003

18 Ivanoff CJ, "Influence of mono- and bicortical anchorage on the integration of titanium implants. A study in the rabbit tibia" 25 : 229-235, 1996

19 Wilson TG Jr, "Implants placed in immediate extraction sites: a report of histologic and histometric analyses of human biopsies" 13 : 333-341, 1998

20 Rosenquist B, "Immediate placement of implants into extraction sockets: implant survival" 11 : 205-209, 1996

21 Clausen C, "Homologous activated platelets stimulate differentiation and proliferation of primary human bone cells" 184 : 68-75, 2006

22 Pankov R, "Fibronectin at a glance" 115 : 3861-3863, 2002

23 Barry EL, "Factor XIIIa-mediated cross-linking of fibronectin in fibroblast cell layers. Cross-linking of cellular and plasma fibronectin and of amino-terminal fibronectin fragments" 264 : 4179-4185, 1989

24 Li M, "Enzymatic degradation behavior of porous silk fibroin sheets" 24 : 357-365, 2003

25 Mannai C, "Early implant loading in severely resorbed maxilla using xenograft, autograft, and plateletrich plasma in 97 patients" 64 : 1420-1426, 2006

26 Dal Pra I, "De novo engineering of reticular connective tissue in vivo by silk fibroin non woven materials" 26 : 1987-1999, 2005

27 Chiapasco M, "Clinical outcomes of GBR procedures to correct peri-implant dehiscences and fenestrations: a systematic review" 20 (20): 113-123, 2009

28 Karageorgiou V, "Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells" 71 : 528-537, 2004

29 Kim KH, "Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration" ELSEVIER SCIENCE BV 120 : 327-339, 2005

30 Cao Y, "Biodegradation of silk biomaterials" 10 : 1514-1524, 2009

31 Mansell JP, "Biochemical evidence for altered subchondral bone collagen metabolism in osteoarthritis of the hip" 36 : 16-19, 1997

32 Turunen T, "Bioactive glass granules and polytetrafluoroethylene membrane in the repair of bone defects adjacent to titanium and bioactive glass implants" 9 : 403-407, 1998

33 Cranin AN, "Autogenous bone ridge augmentation using the mandibular symphysis as a donor" 27 : 43-47, 2001

34 Zhao J, "Apatite-coated silk fibroin scaffolds to healing mandibular border defects in canines" 45 : 517-527, 2009

35 Pietrabissa R, "An in vitro study on compensation of mismatch of screw versus cement-retained implant supported fixed prostheses" 11 : 448-457, 2000

36 Couchourel D, "Altered mineralization of human osteoarthritic osteoblasts is attributable to abnormal type I collagen production" 60 : 1438-1450, 2009

37 Soong HK, "Adverse reactions to virgin silk sutures in cataract surgery" 91 : 479-483, 1984

38 Kania RE, "Addition of fibrin sealant to ceramic promotes bone repair: long-term study in rabbit femoral defect model" 43 : 38-45, 1998

39 Sennerby L, "A new microtomographic technique for non-invasive evaluation of the bone structure around implants" 12 : 91-94, 2001

40 Sennerby L, "A morphometric and biomechanic comparison of titanium implants inserted in rabbit cortical and cancellous bone" 7 : 62-71, 1992