개 정액의 냉동보존 시 k-Carrageenan이 정자 성상에 미치는 영향

다국어 초록 (Multilingual Abstract)

This study was conducted to find out the effect that κ-Carrageenan has on the properties of dog sperm when it was added to the cryoprotectant. Extender basically was contained 1.21 g Trizma base, 0.67 g citric acid, 0.4 g glucose, 0.03 g penicillin G, 0.05 g streptomycin sulfate. Extender1 was added with 0.1%, 0.2%, 0.3%, and 0.5% carrageenan, while extender2 was supplemented with glycerol. After freezing-thawing, the motility, viability, acrosome integrity, apoptosis, and ROS (reactive oxygen specifications) of sperm were measured to analyze the effects of the supplementation of carrageenan. Total Motile (TM), Rapid Progressive Motile (RPM), Medium Progressive Motile (MPM), and Immotile were measured through the CASA system after thawing in 37 degree water. Extender with 0.2% κ-carrageenan (64.26 ± 0.49) was significantly higher than control (40.24 ± 8.27) (p < 0.05). RPMs of extender with 0.1%, 0.2% κ-carrageenan (57.64 ± 6.34, 56.47 ± 1.35) were significantly higher than the other groups (p < 0.05). Acrosome integrity was measured by dyeing to PSA-FITC with an epifluorescence microscope. Normal acrosome ratio of extender with 0.5% κ-carrageenan (61 ± 8.03) was higher than the other groups (p < 0.05). Apoptosis was measured with a FACSCalibur flow cytometer using FITC (FITC Annexin V Apoptosis Detection Kit). Treated groups of κ-carrageenan of 0.1% (0.81 ± 0.05), 0.2% (0.85 ± 0.05) were significantly higer (p < 0.05) than control. Modified SYBR/PI staining was used for determination of viability and DCF staining was used for evaluation of ROS. Viability and ROS were not significantly different from other groups. In conclusion, adding a certain concentration of carrageenan to the extender of cryopreservation, carrageenan contributes to the improvement of the sperm motility, acrosome integrity and prevention of apoptosis.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 공시축
• 정액채취
• 동결정액 제조
• 동결보존액 제조
• 정자 운동성 평가
• 정자 생존율 평가
• 첨체 반응도 분석
• 세포자가사멸율 평가
• 활성산소율(ROS) 평가
• 통계처리
• 결과
• 정자의 운동성 평가
• 정자의 생존율 평가
• 정자의 첨체 온전성 평가
• 세포자가사멸율 평가
• 정자의 활성산소 측정
• 고찰
• CONFLICTS OF INTEREST
• ACKNOWLEDGEMENTS
• REFERENCES

참고문헌 (Reference)

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