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      Effects of Hyaluronidase during In Vitro Maturation on Maturation and Developmental Competence in Porcine Oocytes

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      https://www.riss.kr/link?id=A106970885

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      다국어 초록 (Multilingual Abstract)

      The aim of this study was to investigate effects of hyaluronidase during IVM on oocyte maturation, oxidative stress status, expression of cumulus expansion-related (PTX, pentraxin; GJA1, gap junction protein alpha 1; PTGS2, prostaglandin-endoperoxide ...

      The aim of this study was to investigate effects of hyaluronidase during IVM on oocyte maturation, oxidative stress status, expression of cumulus expansion-related (PTX, pentraxin; GJA1, gap junction protein alpha 1; PTGS2, prostaglandin-endoperoxide synthase 2) and fatty acid metabolism-related (FADS1, delta-6 desaturase; FADS2, delta-5 desaturase; PPARα, peroxisome proliferator-activated receptor-alpha) mRNA, and embryonic development of porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated with 0.1 mg/mL hyaluronidase for 44 h. Cumulus expansion was measured at 22 h after maturation. At 44 h after maturation, nuclear maturation, intracellular glutathione (GSH) and reactive oxygen species (ROS) levels were measured. Gene expression in cumulus cells was analyzed using real time PCR. The cleavage rate and blastocyst formation were evaluated at Day 2 and 7 after insemination. In results, expansion of cumulus cells was suppressed by treatment of hyaluronidase at 22 h after maturation. Intracellular GSH level was reduced by hyaluronidase treatment (p < 0.05). On the other hand, hyaluronidase increased ROS levels in oocytes (p < 0.05). Only PTGS2 mRNA was enhanced in COCs by hyaluronidase (p < 0.05). Population of oocytes reached at metaphase II stage was higher in control group than hyaluronidase treated group (p < 0.05). Both of cleavage rate and blastocyst formation were higher in control group than hyaluronidase group (p < 0.05). Our present results showed that developmental competence of porcine oocytes could be reduce by hyaluronidase via inducing oxidative stress during maturation process and it might be associated with prostaglandin synthesis. Therefore, we suggest that suppression of cumulus expansion of COCs could induce oxidative stress and decrease nuclear maturation via reduction of GSH synthesis and it caused to decrease developmental competence of mammalian oocytes.

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      목차 (Table of Contents)

      • ABSTRACT
      • INTRODUCTION
      • MATERIALS AND METHODS
      • Oocytes collection and in vitro maturation (IVM)
      • Assessment of cumulus cell expansion
      • ABSTRACT
      • INTRODUCTION
      • MATERIALS AND METHODS
      • Oocytes collection and in vitro maturation (IVM)
      • Assessment of cumulus cell expansion
      • Measurement of intracellular GSH and ROS levels
      • Evaluation of nuclear maturation
      • Quantitative real time PCR
      • In vitro fertilization and culture
      • Statistical analysis
      • RESULTS
      • Effects of hyaluronidase on cumulus expansion and oxidative stress status
      • Changes of mRNA expression by hyaluronidase
      • Nuclear maturation and embryonic development
      • DISCUSSION
      • CONFLICTS OF INTEREST
      • ACKNOWLEDGEMENTS
      • ORCID
      • REFERENCES
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