Effect of Lithospermi Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice

다국어 초록 (Multilingual Abstract)

Objectives: The root of Lithospermum erythrorhizon Sieb. et Zucc. (Lithospermi Radix, LR) is a kind of heat clearing and blood cooling medicinal herbs. It can clear away heat and cool the blood, reduce toxins and disperse maculae. LR has long been used as efficacious therapy for inflammation, burns,frostbite and skin diseases such as eczema and psoriasis.
Methods: In the present study, we investigate anti-allergic and anti-inflammatory effects of LR by using the 1-fluoro-2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis mouse model.
Results: Topical application of 10 mg/mL of LR effectively inhibited skin lesions induced by repeated paintings with DNFB.
Topical application of LR also inhibited hyperplasia, edema,spongiosis and infiltrations of mononuclear cells. In addition,production levels of total immunoglobulin and IgG1 in serum were decreased by using LR in vivo.
Conclusions: These data suggest that LR acts as an antiinflammatory agent, improving skin lesions in CD mice.

참고문헌 (Reference)

1 이용섭, "자초 뿌리의 함유성분 및 아토피피부염 관련 연구현황" 한국생약학회 41 (41): 73-88, 2010

2 Cohen D., "Treatment of irritant and allergic contact dermatitis" 17 (17): 334-340, 2004

3 Kyogoku K., "Studies on the constituents of Shikon. II : Comparison of contents, constituents, and antibacterial effect of fat soluble fraction between Nanshikon and Koshikon" 27 (27): 31-36, 1973

4 Niwano Y., "Shikonin, an active principle of the medicinal plant Lithospermum erythrorhizon, is a potent scavenger of peroxyradicals in cigarette smoke, In recent progress in medicinal plants" Studium Press LLC 203-214, 2007

5 Chen X., "Shikonin, a component of Chinese herbal medicine, inhibits chemokine receptor function and suppresses human immunodeficiency virus type 1" 47 (47): 2810-2816, 2003

6 Bickers DR., "Oxidative stress in the pathogenesis of skin disease" 126 (126): 2565-2575, 2006

7 Broide DH., "Molecular and cellular mechanisms of allergic disease" 108 (108): S65-71, 2001

8 Dudeck A., "Mast cells are key promoters of contact allergy that mediate the adjuvant effects of haptens" 34 (34): 973-984, 2011

9 Grabbe S., "Immunoregulatory mechanisms involved in elicitation of allergic contact hypersensitivity" 19 (19): 37-44, 1998

10 Boerrigter GH., "Hapten-specific antibodies in allergic contact dermatitis in the guinea pig" 85 (85): 385-391, 1988

11 Machado-Pinto J., "Eosinophilic and neutrophilic spongiosis : clues to the diagnosis of immunobullous diseases and other inflammatory disorders" 15 (15): 308-316, 1996

12 Wen MC., "Efficacy and tolerability of anti-asthma herbal medicine intervention in adult patients with moderate-severe allergic asthma" 116 (116): 517-524, 2005

13 Kim SH., "Effects of Mori Folium, Arctii Fructus, Lithospermum Erythrorhizon on the anti-allergic response" 21 (21): 71-79, 2005

14 Guo XP., "Clinical trial on the effects of Shikonin mixture on later stage lung cancer" 11 (11): 598-599, 1991

15 Weng XC., "Antioxidant properties of components extracted from puccoon(Lithospermum erythrorhizon Sieb. et Zucc.)" 69 (69): 143-146, 2000

16 Saint-Mezard P., "Allergic contact dermatitis" 14 (14): 284-295, 2004