<P>Systematic toxicological study is still required to fully understand the hazard potentials of gold nanoparticles (AuNPs). Because their biomedical applications are rapidly evolving, we investigated developmental toxicity of AuNPs in an <I&...
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https://www.riss.kr/link?id=A107612264
2013
-
SCI,SCIE,SCOPUS
학술저널
275-288(14쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>Systematic toxicological study is still required to fully understand the hazard potentials of gold nanoparticles (AuNPs). Because their biomedical applications are rapidly evolving, we investigated developmental toxicity of AuNPs in an <I&...
<P>Systematic toxicological study is still required to fully understand the hazard potentials of gold nanoparticles (AuNPs). Because their biomedical applications are rapidly evolving, we investigated developmental toxicity of AuNPs in an <I>in vivo</I> embryonic zebrafish model at exposure concentration ranges from 0.08 to 50mg/l. Exposure of zebrafish embryos to 1.3nm AuNPs functionalized with a cationic ligand, N,N,N-trimethylammoniumethanethiol (TMAT-AuNPs), resulted in smaller malpigmented eyes. We determined that TMAT-AuNPs caused a significant increase of cell death in the eye, which was correlated with an increase in gene expression of <I>p53</I> and <I>bax</I>. Expression patterns of key transcription factors regulating eye development (<I>pax6a</I>, <I>pax6b</I>, <I>otx2</I>, and <I>rx1</I>) and pigmentation (<I>sox10</I>) were both repressed in a concentration-dependent manner in embryos exposed to TMAT-AuNPs. Reduced spatial localization of <I>pax6a</I>, <I>rx1</I>, <I>sox10</I>, and <I>mitfa</I> was observed in embryos by whole-mount <I>in situ</I> hybridization. The swimming behavior of embryos exposed to sublethal concentrations of TMAT-AuNPs showed hypoactivity, and embryos exhibited axonal growth inhibition. Overall, these results demonstrated that TMAT-AuNPs disrupt the progression of eye development and pigmentation that continues to behavioral and neuronal damage in the developing zebrafish.</P>