천연보존료 복합 황금추출물이 유산균발효 마늘추출물의 저장성 및 기능성에 미치는 영향

국문 초록 (Abstract)

본 연구는 유산균발효 마늘추출물의 간 보호 효능에 대해 천연보존료인 복합 황금추출물이 유산균발효 마늘추출물의 간 보호 효능에 미치는 영향 여부를 확인하기 위해 수행되었다. 복합 황금추출물은 H2O2에 의한 산화적 손상에 대한 유산균발효 마늘추출물의 간 보호 효능에 있어서 유의적인 영향을 미치지 않았으며, acetaminophen에 의한 급성 간 손상 동물 모델에서 시료에 첨가된 복합 황금추출물은 유산균 발효 마늘추출물의 간 손상 억제 효능에 있어 유의적인 영향을 미치지 않았다. 이상의 결과들을 종합 고찰할 때 복합 황금추출물은 기능성 원료의 유효성에 영향을 미치지 않으면서 액상제형 건강기능성식품의 안전성을 확보할 수 있는 천연보존제로 판단되었다.

다국어 초록 (Multilingual Abstract)

The natural preservative “complex Scutellaria baicalensis extract (BHC)” contains Scutellaria baicalensis, Glycyrrhiza uralensis (liquorice), Zizyphus jujube (jujube), and Astragalus propinquus (milk vetch root). BHC has been used as a natural preservative for more than 10 years to increase storage duration and quality of food with strong antibacterial activity. BHC has been added into functional foods as a subsidiary ingredient. However, no studies have been performed to test whether or not BHC affects the activity of main functional ingredients. In this study, we tested whether or not BHC has any effect on the hepatoprotective activity of lactic acid-fermented garlic extract (LAFGE) when formulated in a clinical test supplement. H2O2-induced oxidative damage in HepG2 cells was not attenuated by BHC, indicating that BHC had no influence on the protective effect of LAFGE against oxidative damage. Furthermore, BHC had no effect on the hepatoprotective effect of LAFGE against acetaminophen-induced acute liver injury in rats, as indicated by no changes in alanine transaminase and aspartate transaminase levels. In conclusion, BHC, formulated in the clinical test supplement with LAFGE, had no effect on hepatoprotective activity, indicating BHC could be considered as a suitable natural preservative for liquefied functional food materials.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• REFERENCES

참고문헌 (Reference)

1 장재선, "항생제 내성균에 대한 황금 추출물의 항균효과" 한국식품영양학회 24 (24): 708-712, 2011

2 정은봉, "유산균발효마늘의 유기황화합물과 CYP2E1-Transfected HepG2 Cell에서 알코올 유발 세포독성에 미치는 영향" 한국식품영양과학회 42 (42): 342-347, 2013

3 이희섭, "유산균 발효 마늘 추출물의 oleic acid로 유도된 비알코올성 지방간에 대한 개선 효과" 한국식품과학회 46 (46): 762-768, 2014

4 최지휘, "알코올성 지방간을 유발시킨 마우스에서 유산균 발효 마늘추출물의 간 보호 효과" 한국식품영양과학회 43 (43): 1642-1647, 2014

5 Lee SS, "Role of CYP2E1 in the hepatotoxicity of acetaminophen" 271 : 12063-12067, 1996

6 Wang H, "Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader" 27 : 612-616, 1999

7 Fraga CG, "Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices : characterization and comparison with homogenates and microsomes" 4 : 155-161, 1988

8 El-Hassan H, "Involvement of mitochondria in acetaminopheninduced apoptosis and hepatic injury : roles of cytochrome c, Bax, Bid, and caspases" 191 : 118-129, 2003

9 Jaeschke H, "Intracellular signaling mechanisms of acetaminophen-induced liver cell death" 89 : 31-41, 2006

10 Schäfer T, "Inhibition of p53 protects liver tissue against endotoxin-induced apoptotic and necrotic cell death" 17 : 660-667, 2003

11 이희섭, "Hepatoprotective Effects of Lactic Acid-fermented Garlic Extract against Acetaminophen-induced Acute Liver Injury in Rats" 한국식품과학회 25 (25): 867-873, 2016

12 Rahman I, "Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method" 1 : 3159-3165, 2006

13 Cho SH, "Antimicrobial characteristics of Scutellariae Radix extract" 30 : 964-968, 2001