FC 1-5 : Efficacy of asymmetric siRNA targeting androgen receptor for the treatment of androgenetic alopecia = FC 1-5 : Efficacy of asymmetric siRNA targeting androgen receptor for the treatment of androgenetic alopecia

다국어 초록 (Multilingual Abstract)

Background: Asymmetric small interfering RNA (asiRNA) can inhibit expression of a target gene efficiently, and can be a therapeutic option for various diseases. Androgenic alopecia (AGA) is caused by genetics and sensitivity to dihydrotestosterone (DHT), which binds to androgen receptor (AR) and leads to hair loss.
Objectives: To evaluate the therapeutic potential of asiRNA(AR), an asiRNA developed to silence AR gene, for the treatment of AGA.
Methods: Human dermal papilla (DP) cells, C57BL/6 mice, and ex vivo human scalp tissues were stimulated with DHT with or without treatment with asiRNA(AR), and the change in expression of AR as well as other molecules implicated in hair growth were evaluated. Also, the mean diameter of hair bulb and the proportions of telogen hair in ex vivo scalp tissues were determined after exposure to DHT with or without asiRNA(AR).
Results: After treatment with asiRNA(AR) in DP cells, no significant toxicity was observed, and the expression of AR was downregulated. Also, treatment with asiRNA(AR) attenuated DHT-induced increase in IL-6, TGFβ1, and DKK1. In the asiRNA(AR)-treated group, the proportion of telogen hair was lower, and the mean hair bulb diameter was thicker than the control stimulated with DHT only. Local injection of asiRNA(AR) in DHT-induced mice resulted in hair growth promotion.
Conclusion: Downregulation of AR expression and prevention of DHT-induced changes could be achieved with asiRNA(AR). Thus, it could be a therapeutic option for AGA.