Genetic Linkage of Genes for a Thermo-sensitive Genic Male Sterility in Maize: The genetic analysis of an TGMS Line Qiong-6ms in maize

다국어 초록 (Multilingual Abstract)

Thermo-sensitive genic male sterile (TGMS) lines can provide new options for hybrid seed production using "two-line" system. A set of F2 and BC1 populations derived from the cross between Qiong-6ms and Dan958 were employed to analyze the inheritance of a TGMS line Qiong-6ms and map the TGMS genes in maize. The results demonstrated that the sterility of Qiong-6ms was governed by two duplicative recessive genes, named tms1 and tms2. The gene tms1 was mapped to chromosome 5 linked with the SSR markers umc1355, umc2302 and umc1784 at a distance of 3.0 cM, 1.3 cM and 0.9 cM respectively; while tms2 was localized on chromosome 3, linked with SSR markers bnlg1605 (0.5 cM) and umc2050 (4.2 cM). These markers, which are tightly linked with the tms1 and tms2 genes, will be helpful for marker assisted selection of TGMS lines in maize.

참고문헌 (Reference)

1 Tang JH, "The relationship about the fertility conversion mechanism of the thermo-sensitive genic male-sterility in maize and exogenous hormone" 6 : 12-13, 2002

2 Si MS, "The discovery, determination and utilization of the Hubei photo-sensitive genic male-sterile rice (Oryza sativa subsp. Japonica)" 13 : 107-112, 1986

3 He ZY, "The discovery and preliminary study of the temperature-sensitive genic male-sterile maize" 2 : 1-2, 1995

4 Duvick DN, "The chromosomal location of Rf1, a restorer gene for cytoplasmic pollen sterile maize" 46 : 1245-1252, 1961

5 Wang B, "Tagging and mapping the thermo-sensitive genic male-sterile gene in rice with molecular marker" 91 : 1111-1114, 1995

6 Li JX, "Study on the inheritance of cytoplasmic male-sterile and fertility restoration in maize" 2 : 339-362, 1963

7 Tang JH, "Study on the fertility conversion mechanism of the temperature-sensitive genic male-sterile line in maize" 34 : 4-6, 2000

8 He ZY, "Study on ecology of the thermo-sensitive male sterility of corn" 15 : 4-10, 1999

9 Wu WR, "Strategy for the mapping of interactive genes using bulked segregant analysis method and Mapmaker/Exp software" 51 : 2619-2623, 2006

10 Murray MG, "Rapid isolation of high molecular weight plant DNA" 8 : 4321-4325, 1980

11 Subudhi PK, "Molecular mapping of a thermo-sensitive genic male sterility in rice using bulked segregant analysis" 40 : 188-194, 1997

12 Dong NV, "Molecular mapping of a rice gene conditioning thermo-sensitive genic male sterility using AFLP, RFLP and SSR techniques" 100 : 727-734, 2000

13 Koh HJ, "Molecular mapping of a new genic malesterility gene causing chalky endosperm in rice (Oryza sativa L.)" 106 : 57-62, 1999

14 Ku SJ, "Male-sterility of thermo-sensitive genic male-sterile rice is associated with premature programmed cell death of the tapetum" 217 : 559-565, 2003

15 Lander ES, "MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations" 1 : 174-181, 1987

16 Yamagushi Y, "Linkage analysis of the thermo-sensitive genic male sterility gene tms in rice (Oryza sativa L.)" 47 : 371-377, 1997

17 Yuan LP, "Increasing yield potential in rice by exploitation of heterosis. In Hybrid rice technology. New developments and future prospects" IRRI 1-6, 1994

18 Michelmore RW, "Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic region by using segregating population" 88 : 9828-9832, 1991

19 Lee DS, "Genetic characterization and fine mapping of a novel thermo-sensitive genic male-sterile gene tms6 in rice (Oryza sativa L.)" 111 : 1271-1277, 2005

20 Wang B, "Genetic and molecular study of photo-sensitve and thermo-sensitive male sterility in rice. In The theoretical and technical study of two-line hybrid rice" Science Press 36-56, 2001

21 Xing QH, "Genetic analysis, molecular tagging and mapping of the thermo-sensitive genic male-sterile gene (wtms1) in wheat" 107 : 1500-1504, 2003

22 Wang YG, "Fine mapping of the rice thermo-sensitive male-sterile gene tms5" 107 : 917-921, 2003

23 Praba ML, "Effect of growth regulators and chemicals on pollen sterility in TGMS lines of rice" 46 : 117-124, 2005

24 Wang CH, "Development of a genetic marker linked to a new thermo-sensitive male sterile gene in rice (Oryza sativa L.)" 140 : 217-222, 2004

25 Jia JH, "Constructing a genetic linkage map and mapping a new thermo-sensitive genic male-sterile gene (tms5) in rice" 37-, 2000

26 Zhang YD, "Analysis differential expression of thermo-sensitive genic-male-sterility gene in maize" 25 : 66-70, 2004