虛血/再灌流 腎臟 組織에서 覆盆子의 抗酸化 효과 = Effects of Rubi Fructus on damage of rat's kidney induced by ischemia and reperfusion

다국어 초록 (Multilingual Abstract)

Rubi Fructus (RF), being known to reinforce kidney, have tested to study the effects concerning damages of renal tissue induced by oxygen free radicals.
In vitro, the level of lipid peroxide in renal tissue was decreased proportinally according to dose by RF extract. And, it was seen proportinally decrease according to the dose of RF on xanthine oxidase activities and type conversion ratio. And, I had observed the effects of RF extract on damages of rat's kidney following ischemia and reperfusion. Before ischemia was caused, RF extract was applied through mouth in ischemia and reperfusion group and normal saline was applied in normal group. Ischemia was caused by renal artery's clamp for 60 min and reflowed by clamp remove after 30 min. It was increased on the content of lipid peroxidation, activities and type conversion ratio of xanthine oxidase following ischemia and reperfusion. However, they were decreased when RF extract was pre-appllied. Glutathione level was decreased in ischemia and reperfusion group, and increased in RF extract's pre-applied group. After ischemia for 60 min and reflowing for 30 min, I can find the enzyme activities of superoxide dismutase and glutathione peroxidase highly decreased compared to the normal group. However, they were increased in case of RF extract's pre-applied group.
In conclusion, RF extract recovers the damage of kidney due to ischemia and reperfusion by decreasing the lipid peroxidation and xanthine oxidase's activities and by increasing activities of oxygen free radical's scavengeing enzymes.

목차 (Table of Contents)

• 目次
• Ⅰ. 緖論 = 1
• Ⅱ. 材料 및 方法 = 3
• 1. 재료 = 3
• 1) 약재 = 3
• 2) 시약 및 기기 = 3
• 3) 동물 = 3
• 2. 방법 = 3
• 1) 추출물의 제조 = 3
• 2) 處血 後 再灌流 실험 = 4
• 3) 효소원의 조제 = 4
• 4) 효소 활성의 측정 = 6
• ① Xanthine oxidase 활성 측정 = 6
• ② Superoxide dismutase 활성 측정 = 6
• ③ Glutathione peroxidase 활성 측정 = 6
• 5) 과산화지질 함량 측정 = 7
• 6) Glutathione 함량 측정 = 7
• 7) 단백질의 정량 및 통계처리 = 7
• Ⅲ. 實驗成績 = 8
• 1. 시험관내에서 과산화지질 생성에 미치는 영향 = 8
• 2. 시험관내에서 xanthine oxidase 활성에 미치는 영향 = 8
• 3. 시험관내에서 xanthine oxidase 형전환비에 미치는 영향 = 8
• 4. 虛血 後 再灌流에 따른 과산화지질 생성에 미치는 영향 = 8
• 5. 虛血 後 再灌流에 따른 glutathione 함량에 미치는 영향 = 9
• 6. 虛血 後 再灌流에 따른 xanthine oxidase 활성에 미치는 영향 = 9
• 7. 虛血 後 再灌流에 따른 xanthine oxidase 형전환에 미치는 영향 = 9
• 8. 虛血 後 再灌流에 따른 superoxide dismutase 활성에 미치는 영향 = 9
• 9. 虛血 後 再灌流에 따른 glutathione peroxidase 활성에 미치는 영향 = 10
• Ⅳ. 考察 = 20
• Ⅴ. 結論 = 24
• 參考文獻 = 25
• ABSTRACT = 30