조직확장기 응용을 위한 생분해성 탄성 하이드로젤의 제조

다국어 초록 (Multilingual Abstract)

In this study, aof biodegradable block copolymer hydrogels having a delayed swelling property were prepared and evaluated for application as a tissue expander.The hydrogels were synthesized via radical crosslinking reaction of poly(ethylene glycol) (PEG) diacrylate as a hydrophilic macromonomer and poly(d,l-lactide-co-glycolide)-poly(ethylene glycol)-poly(d,l-lactide-co-glycolide)(PLGA-PEG-PLGA) triblock copolymer diacrylate as a swelling/degradation controller (SDC), respectively. For the synthesis of various SDCs that can lead to different degradation and swelling properties, various PLGA-PEG-PLGA triblock copolymers with different LA/GA ratios and different PLGA block lengths were synthesized and wereto have terminal acrylate groups. The resultant hydrogels were flexible and elastic, and thus can beeasily cut with a knife or scissors even in the dry state. This allows easy reshaping of these materials at the time of implantation. The in-vitro degradation tests showed that the delayed swelling properties of the hydrogels could be modulated by varying the chemical composition of the biodegradable crosslinker and thetime before swelling rangedone week to five weeks. The hydrogels could be useful for biomedical applications such as tissue expansion due to their biocompatibility, elasticity, sufficient swelling pressure, delayed swelling and controllable degradation time.

목차 (Table of Contents)

• 1. 서론 1
• 1.1. 하이드로젤 1
• 1.1.1. 서론 1
• 1.1.2. 하이드로젤의 분류 2
• 1.1.3. 하이드로젤의 의약학적 응용 8
• 1.2 생분해성 고분자 9
• 1.2.1. 서론 9
• 1.2.2. 화학가수분해형 생분해성 고분자 11
• 1.2.3. 분해 메커니즘 14
• 1.2.4. 생분해성 폴리에스테르 16
• 1.3. PEG 19
• 1.4 조직확장기 20
• 1.4.1. 서론 20
• 1.4.2. 조직확장기사용의 장단점 22
• 1.4.3. 자가팽창 조직확장기 24
• 1.5. 본 연구의 목적 26
• 2. 실험방법 29
• 2.1. 시약 29
• 2.2. Swelling/Degradation Controller(SDC) 의 합성 29
• 2.2.1. PLGA-PEG-PLGA-DA 29
• 2.2.1.1. PLGA-PEG-PLGA 공중합체의 합성 29
• 2.2.1.2. PLGA-PEG-PLGA diacrylation 30
• 2.2.2. PLA-PEG-PLA-DA 30
• 2.2.2.1. PLA-PEG-PLA 공중합체의 합성 30
• 2.2.2.2. PLA-PEG-PLA diacrylation 31
• 2.2.3. PCL-DA 31
• 2.2.3.1. PCL diacrylation (PCL-DA) 31
• 2.3. Crosslinking agent의 합성 35
• 2.3.1. PEG diacrylation (PEG-DA) 35
• 2.4 하이드로젤의 제조 37
• 2.4.1. PLGA-PEG-PLGA/PEG하이드로젤의 제조 37
• 2.4.2. PLA-PEG-PLA/PEG하이드로젤의 제조 37
• 2.4.3. PCL/PEG 하이드로젤의 제조 37
• 2.5 특성평가방법 39
• 2.5.1 고분자 분석 39
• 2.5.2 기계적 물성 측정 39
• 2.5.3 팽윤압력 측정 40
• 2.5.4 분해 거동 측정 41
• 3. 결과 및 고찰 42
• 3.1. 특성평가 42
• 3.1.1. PLGA-PEG-PLGA/PEG hydrogel 42
• 3.1.2. PLA-PEG-PLA/PEG hydrogel 49
• 3.1.3. PCL/PEG hydrogel 52
• 3.2. 기계적 물성 측정 56
• 3.3. 팽윤압력 측정 60
• 3.4. 분해 거동 측정 62
• 4. 결론 68
• 5. 참고문헌 70