Change in Photosynthetic Pigment, Soluble Protein and Reducing Sugar Contents in Spinach Leaves with Paraquat Treatment under Light = 제초제 Paraquat가 시금치 잎의 광합성 색소, 단백질 및 환원당 함량에 미치는 영향

국문 초록 (Abstract)

제초제 Paraquat가 시금치 잎의 생리활성에 미치는 영향을 구명하기 위하여 재료 식물의 잎을 0.1, 1.0, 10, 20, 50 및 100ppm의 paraquat 용액에 광하에서 24hr 동안 floating하여 광합성 색소, 단백질 및 환원당 함량에 미치는 영향을 조사하였다.
광합성 색소에 미치는 효과는 0.1, 1.0, 10, 20, 50과 100ppm에서 생체 1g당 엽록소 함량 (total chlorophyll) 이 대조군에 비하여 4, 17, 28, 47, 54 및 66%까지 각각 감소하였으며 carotenoid 함량은 8, 32, 42, 40, 49 및 89% 까지 각각 감소 하였다.
단백질 함량과 reducing sugar 함량은 0.1, 1.0, 10, 20, 50 및 100ppm의 paraquat농도의 처리군에는 대조군에 비하여 4, 18, 37, 45, 48 및 62% 와 10, 20, 21, 34, 43 및 48% 까지 감소했다.
따라서 본 실험의 결과 paraquat는 광합성 및 물질대사와 같은 정상적 대사과정을 저해함으로서 제초제의 효과를 나타내는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

The objective of this study was to investigate the change in photosynthetic pigment, soluble protein and reducing sugar contents in leaf disc with paraquat treatment under light.
Leaf discs were excised from spinach leaves(Spinacia oleracea L.) and floated in phosphate buffer (pH 6.8) containing paraquat solutions (0, 0.1, 1.0, 10, 20, 50and 100 ppm) and incubated in the growth chamber under 5,500 Lux illumination at 25℃ for 24 hr.
When 0.1, 1.0, 10, 20, 50 and 100 ppm of paraquat solutions were applied to leaf discs, the percentages of decrease in the contents of total chlorophyll were 4, 17, 28, 47, 54 and 66% of initial level, respectively and those in the contents of carotenoid were 8, 32, 42, 40, 49 and 89% of initial level, respectively.
The contents of soluble protein in 0.1, 1.0, 10, 20, 50 and 100 ppm of paraquat-treated groups were reduced to 4, 18, 37, 45, 48 and 62%, respectively, the contents of reducing sugar to 10, 20, 21, 34, 43 and 48%, respectively as compared to control group.
These results suggest that paraquat triggers the block of chlorophyll biosynthesis and the destruction of existing pigments, the reduction of reducing sugar and the decrease of protein content. Paraquat has the inhibitory effects on the process and pathway of plant metabolism.

목차 (Table of Contents)

• Ⅰ. Introduction
• Ⅱ. Materials and Methods
• 1. Plant materials
• 2. Paraquat treatments and incubation
• 3. Measurement of photosynthetic pigment contents
• 4. Measurement of soluble protein contents
• 5. Measurement of reducing sugar contents
• Ⅲ. Results
• 1. Photosynthetic pigment contents
• 2. Soluble protein contents
• 3. Reducing sugar contents
• Ⅳ. Discussion
• References
• Abstract