시중 유통 막걸리의 유기산 조성과 생리활성

국문 초록 (Abstract)

막걸리는 고려시대 이후 제조된 우리나라 전통술이다. 본 연구는 막걸리의 유기산 분석과 생리 기능활성을 평가하고자 수행하였다. 막걸리의 유기산 분석 결과, 막걸리는 유기산 중에서 유산균에 의한 대사 산물인 lactic acid를 가장 많이 함유하고 있었으며 시판 막걸리에 따라 다양한 유기산 조성을 나타내고 있었다. 막걸리의 항비만 효과를 전지방세포인 3T3-L1 세포주를 사용하여 평가한 결과, 대조군 대비 막걸리는 전지방세포 분화를 저해하였으며, 특히 100 μg/mL의 농도에서 40-70% 수준으로 분화를 억제하였다. 또한 혈관신생 억제 효과와 항염증 활성을 관찰하기 위하여, 각각 CAM assay와 LPS를 처리하여 염증반응을 유발한 murine macrophage RAW264.7 세포주에서 NO를 정량하였다. 그 결과, 대부분의 막걸리는 50% 이상의 혈관신생 억제 효과를 확인하였다. 또한 농도의존적으로 NO의 생성량을 유의적 수준으로 저해하였다. 이런 결과를 종합할 때, 막걸리는 항비만, 혈관신생 억제, 그리고 항염증 활성을 가지고 있으며, 이는 막걸리의 누룩 미생물 균총의 2차 대사산물의 효과에 기인한다고 생각된다.

다국어 초록 (Multilingual Abstract)

Makgeolli is Korean traditional alcoholic beverage that has historically been brewed. In this study, we analyzed the profile of organic acids in makgeolli and also evaluated its physiological characteristics. Makgeolli contained excess lactic acid, which is produced by lactic acid bacteria (LAB). Anti-obesity effects of makgeolli were investigated in 3T3-L1 preadipocytes. Compared to the negative control, makgeolli inhibited the differentiation of preadipocyte as quantified by Oil red O dye. In particular, 100 μg/mL makgeolli reduced 40 to 70% of differentiation. To evaluate the anti-angiogenic and anti-inflammatory effects of makgeolli, we performed chorioallantoic membrane assay and measured nitric oxide production from lipopolysaccharide-induced RAW264.7 cells. Most makgeolli interrupted the formation of neo-vasculature and significantly inhibited NO production in a dose-dependent manner. Taken together, these findings suggest that commercial makgeolli has inhibitory activities against adipogenesis, neo-vascularization, and inflammation, and also they
are influenced by second metabolites from nuruk microflora containing fungi and LAB.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

참고문헌 (Reference)

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