Desorption and reusability of adsorbent Diaion HP-20 for the recovery of paclitaxel from culture supernatants of Taxus chinensis

다국어 초록 (Multilingual Abstract)

In this study, an ultrasonic desorption process was developed for the efficient recovery of paclitaxel from Taxus chinensis culture supernatants using a commercial adsorbent, Diaion HP-20. In addition, the reusability of the adsorbent was investigated. The optimal ultrasonic power, operating time, adsorbent/solvent ratio, temperature, solvent type, and concentration for ultrasonic desorption were 180 W, 20 min, 1:30 w/v, 25 °C, methanol, and 100%, respectively. Furthermore, all of the paclitaxel could be recovered after three desorption cycles. The high bubble collapse intensity of acoustic cavitation played a more decisive role than the high bubble density of hydrodynamic cavitation. Diaion HP-20 maintained a high adsorption efficiency of over 95% even after five regenerations, and the stability of the adsorbent was confirmed through electron microscopy, scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy analyses.