Comparative triple-color FISH mapping in eleven Senna species using rDNA and telomeric repeat probes

다국어 초록 (Multilingual Abstract)

Senna is a diverse and paraphyletic genus in the subfamily Caesalpinioideae (Fabaceae Lindl.) comprising various speciesof industrial and medicinal value. To date, the genome-based taxonomic relationship among several Senna species remainsenigmatic. Cytogenetic information is invaluable in deciphering phylogenetic relationships and evolutionary history. However,insuffi cient chromosomal research for many Senna species impedes comparative cytotaxonomic analyses aimed at understandingtheir genomic evolution. To provide additional Senna -related molecular cytogenetic information, we karyotyped11 Senna species by employing triple-color fl uorescence in situ hybridization using 5S rDNA, 45S rDNA, and Arabidopsisthaliana -type telomeric pre-labeled oligonucleotide probes. Chromosome numbers were predominantly 2 n = 28, but 2 n = 22( S. marilandica ) and 2 n = 24 ( S. unifl ora ) were also observed. While most species revealed only one interstitial 5S rDNAlocus, except for S. unifl ora which has two loci, a range of one to three 45S rDNA loci were detected at distal chromosomalregions. Additionally, we observed a hemizygous 45S rDNA locus in S. auriculata . In addition to chromosome termini,weak signals for telomeric repeats were found in interstitial regions in S. hirsuta, S. corymbosa , and S. alexandrina . Thesecytogenetic data can be integrated with molecular phylogenetic data for more comprehensive Senna cytotaxonomic analyses.

참고문헌 (Reference)

1 Remnyl Joyce Pellerin, "Triple-color FISH Karyotype Analysis of Four Korean Wild Cucurbitaceae Species" 한국원예학회 36 (36): 98-107, 2018

2 A. I. Shchapova, "The diversity of lifecycles and their role in the evolution of basic chromosome numbers in various living species" Pleiades Publishing Ltd 3 (3): 239-245, 2013

3 Anna Rice, "The chromosome counts database (CCDB) - a community resource of plant chromosome numbers" Wiley 206 (206): 19-26, 2015

4 A. Ruiz-Herrera, "Telomeric repeats far from the ends: mechanisms of origin and role in evolution" S. Karger AG 122 (122): 219-228, 2008

5 J. Fuchs, "Telomere sequence localization and karyotype evolution in higher plants" Springer Science and Business Media LLC 196 (196): 227-241, 1995

6 Nomar Espinosa Waminal, "Repeat Evolution in Brassica rapa (AA), B. oleracea (CC), and B. napus (AACC) Genomes" 한국육종학회 4 (4): 107-122, 2016

7 Nomar Espinosa Waminal, "Rapid and Efficient FISH using Pre-Labeled Oligomer Probes" Springer Science and Business Media LLC 8 (8): 1-10, 2018

8 Hui Chao Zhou, "Pre-labelled oligo probe-FISH karyotype analyses of four Araliaceae species using rDNA and telomeric repeat" 한국유전학회 41 (41): 839-847, 2019

9 Kátia RESENDE, "Polyploidy and apomixis in accessions of Senna rugosa (G.Don) H.S.Irwin & Barneby" The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) 38 : 510-515, 2014

10 J. Maluszynska, "Physical mapping of rDNA loci in Brassica species" Canadian Science Publishing 36 (36): 774-781, 1993

11 B. Marazzi, "Phylogenetic relationships within Senna (Leguminosae, Cassiinae) based on three chloroplast DNA regions: patterns in the evolution of floral symmetry and extrafloral nectaries" Wiley 93 (93): 288-303, 2006

12 Vratislav Peska, "Origin, Diversity, and Evolution of Telomere Sequences in Plants" Frontiers Media SA 11 : 117-, 2020

13 M. Oliur Rahman, "Numerical taxonomy of the genus Senna Mill. from Bangladesh" Bangladesh Journals Online (JOL) 20 (20): 77-83, 2013

14 Fernando Roa, "Non-Random Distribution of 5S rDNA Sites and Its Association with 45S rDNA in Plant Chromosomes" S. Karger AG 146 (146): 243-249, 2015

15 ALBERT LEVAN, "NOMENCLATURE FOR CENTROMERIC POSITION ON CHROMOSOMES" Springer Science and Business Media LLC 52 (52): 201-220, 2009

16 Caroline A Schneider, "NIH Image to ImageJ: 25 years of image analysis" Springer Science and Business Media LLC 9 (9): 671-675, 2012

17 조영관, "Morphological Characteristics and FISH Analysis of Hibiscus F1 Hybrids and Parental Lines" 한국원예학회 37 (37): 630-639, 2019

18 Dal-Hoe Koo, "Molecular cytogenetic mapping of Cucumis sativus and C. melo using highly repetitive DNA sequences" Springer Science and Business Media LLC 18 (18): 325-336, 2010

19 Remnyl Joyce Pellerin, "Karyotypes of Three Exotic Cucurbit Species Based on Triple-Color FISH Analysis" 한국원예학회 36 (36): 417-425, 2018

20 J. G. Seijo, "Karyotype analysis and chromosome evolution in South American species of Lathyrus (Leguminosae)" Wiley 90 (90): 980-987, 2003

21 Joel Maciel Pereira Cordeiro, "Intra- and interspecific karyotypic variations of the genus Senna Mill. (Fabaceae, Caesalpinioideae)" FapUNIFESP (SciELO) 32 (32): 128-134, 2018

22 Gustavo Souza, "Interstitial telomeric sites and Robertsonian translocations in species of Ipheion and Nothoscordum (Amaryllidaceae)" Springer Science and Business Media LLC 144 (144): 157-166, 2016

23 Li He, "Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species" Springer Science and Business Media LLC 21 (21): 5-13, 2013

24 Wakana Uchida, "Interstitial telomere-like repeats in the Arabidopsis thaliana genome." Genetics Society of Japan 77 (77): 63-67, 2002

25 Ingo Schubert, "Interpretation of karyotype evolution should consider chromosome structural constraints" Elsevier BV 27 (27): 207-216, 2011

26 Marcela Rosato, "Inter- and intraspecific hypervariability in interstitial telomeric-like repeats (TTTAGGG)n in Anacyclus (Asteraceae)" Oxford University Press (OUP) 122 (122): 387-395, 2018

27 Hui Chao Zhou, "In silico mining and FISH mapping of a chromosome‑specific satellite DNA in Capsicum annuum L." 한국유전학회 41 (41): 1001-1006, 2019

28 Kang SH, "Genome-enabled discovery of anthraquinone biosynthesis in Senna tora" 11 : 5875-, 2020

29 Alexandra Wölk, "Genome evolution in a Mediterranean species complex: phylogeny and cytogenetics ofHelictotrichon(Poaceae) allopolyploids based on nuclear DNA sequences (rDNA, topoisomerase gene) and FISH" Informa UK Limited 13 (13): 326-345, 2015

30 Jan Vrána, "Flow cytometric chromosome sorting in plants: The next generation" Elsevier BV 57 (57): 331-337, 2012

31 Remnyl Joyce Pellerin, "FISH mapping of rDNA and telomeric repeats in 10 Senna species" 한국원예학회 60 (60): 253-260, 2019

32 Eliazar Alumbro Peniton Jr., "FISH Karyotype Comparison between Wild and Cultivated Perilla Species Using 5S and 45S rDNA Probes" 한국육종학회 7 (7): 237-244, 2019

33 Winterfeld G, "Dysploidy and polyploidy trigger strong variation of chromosome numbers in the prayer-plant family (Marantaceae)" 2020

34 Tianying Lan, "Dynamic distribution patterns of ribosomal DNA and chromosomal evolution in Paphiopedilum, a lady's slipper orchid" Springer Science and Business Media LLC 11 (11): 126-, 2011

35 Nomar Espinosa Waminal, "Dual-color FISH Karyotype and rDNA Distribution Analyses on Four Cucurbitaceae Species" 한국원예학회 53 (53): 49-56, 2012

36 Franklin Hinosa Mancia, "Distribution of Various Types of Repetitive DNAs in Allium cepa L. Based on Dual Color FISH" 한국원예학회 56 (56): 793-799, 2015

37 Fernando Roa, "Distribution of 45S rDNA sites in chromosomes of plants: Structural and evolutionary implications" Springer Science and Business Media LLC 12 (12): 225-, 2012

38 Lei Chen, "Development of a set of chromosome-specific oligonucleotide markers and karyotype analysis in the Japanese morning glory Ipomoea nil" Elsevier BV 273 : 109633-, 2020

39 MARIA GORETI C. SOUZA, "Cytogenetics and chromosome banding patterns in Caesalpinioideae and Papilionioideae species of Pará, Amazonas, Brazil" Oxford University Press (OUP) 144 (144): 181-191, 2004

40 Biondo Elaine, "Cytogenetics and Cytotaxonomy of Brazilian Species of Senna Mill. (Cassieae - Caesalpinioideae - Leguminosae)" Firenze University Press 58 (58): 152-163, 2005

41 D. Ohri, "Correlations between 2C DNA values and habit inCassia (Leguminosae:Caesalpinioideae)" Springer Science and Business Media LLC 153 (153): 223-227, 1986

42 Monique Mantovani, "Conserved 5S and variable 45S rDNA chromosomal localisation revealed by FISH in Astyanax scabripinnis (Pisces, Characidae)" Springer Science and Business Media LLC 123 (123): 211-216, 2005

43 J. Matthew Watson, "Comparative biology of telomeres: Where plants stand" Wiley 584 (584): 3752-3759, 2010

44 Thanh Dat Ta, "Comparative FISH analysis of Senna tora tandem repeats revealed insights into the chromosome dynamics in Senna" 한국유전학회 43 (43): 237-249, 2021

45 Aretuza Sousa, "Combining FISH and model-based predictions to understand chromosome evolution in Typhonium (Araceae)" Oxford University Press (OUP) 113 (113): 669-680, 2014

46 William A Freyman, "Cladogenetic and Anagenetic Models of Chromosome Number Evolution: A Bayesian Model Averaging Approach" Oxford University Press (OUP) 67 (67): 195-215, 2018

47 M. Guerra, "Chromosome numbers in plant cytotaxonomy: concepts and implications" S. Karger AG 120 (120): 339-350, 2008

48 MARÍA C. SILVESTRI, "Chromosome diversity in species of the genus Arachis, revealed by FISH and CMA/DAPI banding, and inferences about their karyotype differentiation" FapUNIFESP (SciELO) 92 (92): e20191364-, 2020

49 윤성민, "Chromosome Karyotyping of Senna covesii and S. floribunda based on Triple-color FISH Mapping of rDNAs and Telomeric Repeats" 한국육종학회 6 (6): 51-56, 2018

50 Waminal NE, "Chromosomal mapping of tandem repeats revealed massive chromosomal rearrangements and insights into Senna tora dysploidy" 2021

51 Kuniaki Watanabe, "Chromosomal Cytology and Evolution in Eupatorieae (Asteraceae)" JSTOR 82 (82): 581-592, 1995

52 H. S. Irwin, "CHROMOSOMAL RELATIONSHIPS AND TAXONOMIC CONSIDERATIONS IN THE GENUS CASSIA" Wiley 47 (47): 309-318, 1960

53 Anna Y. Aksenova, "At the Beginning of the End and in the Middle of the Beginning: Structure and Maintenance of Telomeric DNA Repeats and Interstitial Telomeric Sequences" MDPI AG 10 (10): 118-, 2019

54 Ingo Schubert, "Alteration of basic chromosome number by fusion–fission cycles" Canadian Science Publishing 38 (38): 1289-1292, 1995

55 BR Randell, "Adaptations in the genetic system of Australian arid zone Cassia species (Leguminosae, Caesalpinioideae)" CSIRO Publishing 18 (18): 77-97, 1970

56 Turki A. Al-Turki, "A cytological study of flowering plants from Saudi Arabia" Botanic Garden and Botanical Museum Berlin, Freie Universitaet Berlin 30 (30): 339-358, 2000

57 Jerry M. Baskin, "A comparative study of seed dormancy and germination in an annual and a perennial species of Senna (Fabaceae)" Cambridge University Press (CUP) 8 (8): 501-512, 1998