This study were conducted to provide the basic information for the selection of salt and alluminum tolerant lines in Alfalfa (Medicago sativa L.) and Italian ryegrass (Lolium multiflorum L.) by tissue culture. Cotyledon, hypocotyl and roots of alfalfa...
This study were conducted to provide the basic information for the selection of salt and alluminum tolerant lines in Alfalfa (Medicago sativa L.) and Italian ryegrass (Lolium multiflorum L.) by tissue culture. Cotyledon, hypocotyl and roots of alfalfa were cultured on the Blaydes medium, first node and roots of Italian ryegrass were cultured on the MS medium to induce callus. Single cell and callus were selected on the medium containing different levels of NaCl and AICl3.
Germination rate of seeds were decreased ,as the increase of NaCl and AICl3 levels, and severe aslt injuring were occur설 above the 0.5% NaCl level. Alfalfa showed higher cal]us induction rate than Italian ryegrass. Callus induction rate were high in hypocotyl of alfalfa and first node of Italian ryegrass, but callus growth after induction in cotyledon of alfalfa was better than those of hypocotyl. Selection rate of NaCl and AlCl3 tolerant callus became high after the suspension culture. 0.5% NaCl and 200㎛ AIC3 were available concentration for screening salt and alluminum tolerant callus Na+ in callus was increased as the increasing NaCl level on the medium, K+ in the callus was decreased. Selected NaCl tolerant callus show high in Na+, K+ and proline contents compare to nonselected callus,. Proline and alluminum contents in the callus were good indicators for the selection of salt and alluminum tolerant lines.