A two-photon ratiometric probe for detection of hNQO1 enzyme activity in human colon tissue

다국어 초록 (Multilingual Abstract)

<P><B>Abstract</B></P> <P>Human colonic carcinomas are one of the most common cancers in all Western countries, but there is no reliable method for quantitative and rapid analysis of colon cancer. Herein, we report an hNQO1 enzyme-selective and two-photon emission ratiometric probe (<B>SHC</B>) for quantitative analysis of hNQO1 activity in human colon tissue. This probe shows a blue-to-yellow emission color change in response to hNQO1, high sensitivity and selectivity for hNQO1 over other bio-metabolites, and two-photon microscopy (TPM) imaging capability, thereby allowing precise monitoring of the activity of hNQO1 in situ. Ratiometric TPM imaging with <B>SHC</B> in human colon tissues revealed that hNQO1 activities gradually increased during cancer progression from normal to adenoma, and to adenocarcinoma colon tissues. These findings demonstrated that <B>SHC</B> labeling can detect the differential activities of hNQO1 in the distinct stages of colon neoplasm. Thus, this approach, enzyme-selective TP probe labeling, will likely provide a reliable and fast method for precise diagnosis of colon cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-photon emission ratiometric probe for hNQO1 activity was developed. </LI> <LI> The probe shows high sensitivity and selectivity for hNQO1 over other bio-metabolites. </LI> <LI> Quantitative analysis of hNQO1 activity in human colon tissues was demonstrated. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>