Streptozotocin 유도 당뇨 백서에서 경부 림프절과 가지 돌기 세포 변화에 관한 연구 = A STUDY ABOUT THE CHANGE OF CERVICAL LYMPH NODE AND DENDRITIC CELL IN STREPTOZOTOCIN INDUCED DIABETIC RAT

다국어 초록 (Multilingual Abstract)

Diabetes is a metabolic disease, leading to high blood glucose levels, and oral complications were as follows ; cheilosis and a tendency toward drying cracking ; burning sensations : decrease in salivary flow ; alterations in the flora of the oral cavity, with greater predominance of Candida albicans : and increased periodontal disease. The increased susceptibility of diabetics to infection has been well known. The cellular and humoral immune systems also require accessory cells, such as macrophages and antigen-presenting cells, for an optimal response to occur. Male rats weighing about 150-200 gm were induced to diabetes by streptozotocin, and were serially sacrificed on the 1st, 2nd, 3rd day and 1st, 2nd, 6th week of experiment. And then histologic examination on the cervical lymph node & S-100 protein positive dendritic cell were carried out under th light microscope.
The results of this study were as follows :
1. In control group, cervical lymph node consists of a capsule, cortex, paracortex, medullary sinus and medullary cord. The germinal centers appear in the cortex. The medullary cord was composed of closely packed lymphocytes, and the medullary sinus was full of lymph. In the features of immunohistochemical stain, S-100 protein positive cells were seen in the germinal center.
2. In the 1 day experimental group, the germinal centers were more smaller than that of control group and most of them disappeard. The medullary cord was composed of loosely packed lymphocytes. In the features of immunohistochemical stain, S-100 positive protein cells were decreased and somewhere, negative.
3. In the 3 day experimental group, it is similar to 1 day finding but the germinal center had clear margin than that of 1 day experimental group. In the feature of immunohistochemical stain, the number of S-100 protein positive cell was decreased and somewhere, negative.
4. In the 1 week experimental group. the germinal center was more clear than that of 3 day experimental group and T lymphocytes were scattered in the paracortex. In the medullary cord, density of lymphocyte was more increased. In the immunohistochemical stain, S-100 protein positive dendritic cells were increased in the margin of germinal center and somewhere, negative.
5. In the 2 week experimental group, the germinal centers were more recovered. In the medullary cord, density of lymphocyte was increased. In the immunohistochemical stain, S-100 protein positive dendritic cell were increased in the germinal center.
6. In the 6 week experimental group, histologic pattern of cortex and paracortex was similar to control group finding, in the immunohistochemical stain, somewhere, the number of S-100 protein positive dendritic cell was similar to control group.
From above results, in the streptozotocin induced diabetic rat, as the germinal center of cervical lymph node were decreased and antigen-presenting cell, dendritic cell was affected, the function of cervical lymph node, which was responsible for maxillofacial immune system, was decreased.

목차 (Table of Contents)

• Abstract
• I. 서론
• II. 연구재료 및 방법
• III. 연구결과
• IV. 총괄 및 고찰
• V. 결론
• 참고문헌