Perilipin 단백질과 근육 내 중성지방의 상호작용이 PPARβ/δ발현에 미치는 효과

다국어 초록 (Multilingual Abstract)

PURPOSE: We investigated interaction of perilipin protein and intramuscular triglyceride on expression of PPARβ/δin rat skeletal muscle.
METHOD: A total of thirty-six male Sprague-Dawley were used in the study. Rats were divide into three groups: control (n=12), fasted (n=12), aerobic exercise (n=12). Aerobic exercise consist of 2-h (30 min × 4 cycle) swimming exercise and fasting included 18-h with only water. We determined intramuscular triglyceride (IMTG) content, perilipin (PLIN3, PLIN5) and PPARβ/δprotein expression, citrate synthase (CS), β-hydroxyacyl-CoA dehydrogenase (β-HAD) activity.
RESULTS: Our analyses indicate that acute aerobic exercise and fasting unaltered IMTG content both soleus and white gastrocnemius muscle. Skeletal muscle PLIN3, PLIN5, PPARβ/δexhibited a less response to experimental conditions. CS and β-HAD activity remained unaffected.
CONCLUSIONS: These data indicate that exercise stimulus insufficient to improved lipid metabolic capacity. The metabolic health benefits of exercise training to be fully elucidated in older and clinical populations.

목차 (Table of Contents)

• Abstract
• Ⅰ. 서론
• Ⅱ. 연구방법
• Ⅲ. 결과
• Ⅳ. 논의
• Ⅴ. 결론
• 참고문헌

참고문헌 (Reference)

1 Kiens, B., "Types of carbohydrate in an ordinary diet affect insulin action and muscle substrates in humans" 63 : 47-53, 1996

2 Shepherd S. O., "Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure" 595 (595): 5587-5601, 2017

3 Vigelsø, A., "The relationship between skeletal muscle mitochondrial citrate synthase activity and whole body oxygen uptake adaptations in response to exercise training" 6 (6): 84-, 2014

4 Shepherd, S. O., "Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5" 591 (591): 657-675, 2013

5 Amati, F., "Skeletal muscle triglycerides, diacylglycerols, and ceramides in insulin resistance:another paradox in endurance-trained athletes?" 60 (60): 2588-2597, 2011

6 Covington, J. D., "Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation" 9 (9): 1-8, 2014

7 Goodpaster, B. H., "Skeletal muscle lipid content and insulin resistance : Evidence for a paradox in endurance-trained athletes" 86 (86): 5755-5761, 2001

8 Hargreaves, E., "Skeletal muscle energy metabolism during exercise" 2 (2): 817-828, 2020

9 De Lange, P., "Sequential changes in the signal transduction responses of skeletal muscle following food deprivation" 20 (20): 2579-2581, 2006

10 Lee, J. S., "Saturated, but not n-6 polyunsaturated, fatty acids induce insulin resistance : role of intramuscular accumulation of lipid metabolites" 100 (100): 1467-1474, 2006

11 Coen, P. M., "Role of intramyocelluar lipids in human health" 23 (23): 391-398, 2012

12 Russell, A. P., "Regulation of metabolic transcriptional co-activators and transcription factors with acute exercise" 19 (19): 1-20, 2005

13 Spriet, L. L., "Rat skeletal muscle triacylglycerol utilization during exhaustive swimming" 63 : 614-618, 1985

14 Escher, P., "Rat PPARs : quantitative analysis in adult rat tissues and regulation in fasting and refeeding" 42 (42): 4195-4202, 2001

15 Koh, H. C. E., "Pronounced limb and fibre type differences in subcellular lipid droplet content and distribution in elite skiers before and after exhaustive exercise" 595 (595): 5781-5795, 2017

16 Paschoal, V. A., "Positive Reinforcing Mechanisms between GPR120 and PPARγ Modulate Insulin Sensitivity" 31 (31): 1173-1188, 2020

17 Itabe, H., "Perilipins : a diversity of intracellular lipid droplet proteins" 16 (16): 1-11, 2017

18 Laurens, C., "Perilipin 5 fine-tunes lipid oxidation to metabolic demand and protects against lipotoxicity in skeletal muscle" 6 : 1-11, 2016

19 Koves, T. R., "PPARγ coactivator-1α contributes to exercise-induced regulation of intramuscular lipid droplet programming in mice and humans" 54 (54): 522-534, 2013

20 Hardie, D. G., "Organismal carbohydrate and lipid homeostasis" 4 (4): 1-17, 2012

21 Peters, S. J., "Muscle fiber type comparison of PDH kinase activity and isoform expression in fed and fasted rats. American Journal of Physiology-Regulatory" 280 (280): 661-668, 2001

22 Seibert, J. T., "Muscle Lipid Droplets : Cellular Signaling to Exercise Physiology and Beyond" 31 (31): 928-938, 2020

23 Fritz, T., "Low-intensity exercise increases skeletal muscle protein expression of PPARdelta and UCP3 in type 2 diabetic patients" 22 (22): 492-498, 2006

24 Nielsen, J., "Lipid droplet size and location in human skeletal muscle fibers are associated with insulin sensitivity" 313 (313): 721-730, 2017

25 Bosma, M., "Lipid droplet dynamics in skeletal muscle" 340 (340): 180-186, 2016

26 Bergman, B. C., "Intramuscular triglyceride synthesis : importance in muscle lipid partitioning in humans" 314 (314): 152-164, 2018

27 Bindesbøll, C., "Fatty acids regulate perilipin5 in muscle by activating PPARδ" 54 (54): 1949-1963, 2013

28 Strauss, J. A., "Divergence exists in the subcellular distribution of intramuscular triglyceride in human skeletal muscle dependent on the choice of lipid dye" 154 : 369-382, 2020

29 Gemmink, A., "Decoration of intramyocellular lipid droplets with PLIN5 modulates fasting-induced insulin resistance and lipotoxicity in humans" 59 (59): 1040-1048, 2016

30 Delp, D. D., "Composition and size of type I, IIA, IID/X, and IIB fibers and citrate synthase activity of rat muscle" 80 (80): 261-270, 1996

31 Le Garf, S., "Complementary Immunometabolic Effects of Exercise and PPAR β/δ Agonist in the Context of Diet-Induced Weight Loss in Obese Female Mice" 20 (20): 1-19, 2019

32 Phua, W. T. T., "An aPPARent Functional Consequence in Skeletal Muscle Physiology via Peroxisome Proliferator-Activated Receptors" 19 (19): 1-28, 2018

33 Alsted, T. J., "Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training" 296 (296): 445-453, 2009