蓮根 中의 Vitamin B₁分解因子에 關하여 = Studies on the Thiamin Decomposing Factor of Lotus root

국문 초록 (Abstract)

Vitamin B₁수용액에 蓮根을 넣었을 때의 그 농도에 의한 영향 pH에 의한 영향 반응기간·反應溫度에 의한 영향 및 酵素를 不活性하였을 때의 Vitamin B₁의 分解率을 P-Aminoacetophenone method에 의하여 定量하였다. 또한 Paper chromatography method에 의하여 Polyphenol를 分離하여 檢出하였다. 蓮根농도에 따라서 Vitamin B₁의 分解率은 增加되었으며 pH는 3∼6에서 安定하였으나 pH3以下 6 以上에서는 Vitamin B₁의 파괴가 심하였다. 또한 反應時間과 反應溫度에 의한 영향은 別變化가 없었으며 蓮根 중의 酵素를 不活性化시킨 다음 Vitamin B₁용액 一定量에 作用시킨 바 가열을 하지 않은 연근과 거의 같은 結果로 나타난 것으로 미루어 酵素作用은 없는 것으로 推測하는 바이다. Paper chromatography method에 의하여 Polyphenol를 分離하여 세종류의 Chromatogram을 얻었으며, 상기 몇 가지 結果로 미루어 O-diphenol를 가진 Tannin류로 인하여 Vitamin B₁이 감소된 것으로 推定하는 바이다.

다국어 초록 (Multilingual Abstract)

In this study, the thiamin decomposing factor of lotus root dissolved in the aqueous solution of Vitamin B₁, the disintegration rate of the Vitamin, and the effects of the concentration of the lotus root, pH of the solution, reaction time, reaction temperature and enzyme inactivation were determined by the P-Aminoacetophenone method. Polyphenol in the lotus root was detected by paper chromatographic method.
It was found that the disintegration rate of Vitamin B₁increased as the lotus root concentration increased. It was also found that at pH 3-6, Vitamin B₁was stable but at lower than pH3 and at higher than pH6 Vitamin B₁showed a high disintegration rate. The temperature and reaction time produced little effects. On the disintegration of Vitamin B₁.
No enzyme activity seemed to take place, considering the fact that the result obtained from adding into the aqueous solution of Vitamin B₁inactivated enzyme of lotus root was almost the same as that of the unheated lotus root.
Three kinds of chromatogram were obtained from isolating polyphenol by the paper chromatography method.
From these results of the study, it seems that tannins containing O-Diphenol decrease Vitamin B₁

목차 (Table of Contents)

• Ⅰ. 緖 論
• Ⅱ. 實驗方法
• Ⅲ. 實驗結果 및 考察
• Ⅳ. 要 約