Highly sensitive glucose biosensor using new glucose oxidase based biocatalyst

다국어 초록 (Multilingual Abstract)

Glucose, which is a primary energy source of living organisms, can induce diabetes or hypoglycemia if its concentration in blood is irregular. It is therefore important to develop glucose biosensor that reads the concentration of glucose in blood precisely. In the present work, we suggest new glucose oxidase (GOx) based catalysts that can improve the sensitivity of the glucose biosensor and make glucose measurements over a wide concentration ranges possible. For synthesizing such catalysts, a composite including pyrenecarboxaldehyde (PCA) and GOx is attached to substrate including carbon nanotube (CNT) and polyethyleneimine (PEI) (CNT/PEI/[PCA/GOx]). Catalytic activity and stability of the catalyst are then evaluated. According to the investigation, the catalyst shows excellent glucose sensitivity of 47.83 μAcm−2mM−1, low Michaelis-Menten constant of 2.2mM, and wide glucose concentration detection, while it has good glucose selectivity against inhibitors, such as uric acid and ascorbic acid. Also, its activity is maintained to 95.7% of its initial value even after four weeks, confirming the catalyst is stable enough. The excellence of the catalyst is attributed to hydrophobic interaction, C=N bonds, and π-hydrogen interaction among GOx, PCA and PEI/ CNT. The bindings play a role in facilitating electron transport between GOx and electrode.

참고문헌 (Reference)

1 J. Wang, 108 : 814-, 2008

2 K. Hyun, 286 : 197-, 2015

3 Y. Chung, 337 : 152-, 2017

4 M. Christwardana, 9 : e386-, 2017

5 B. Unnikrishnan, 39 : 70-, 2013

6 B. -Y. Wu, 22 : 838-, 2007

7 G. Liu, 8 : 251-, 2006

8 X. Kang, 25 : 901-, 2009

9 L. C. Clark Jr., 102 : 29-, 1962

10 S. Cosnier, 14 : 443-, 1999

11 S. D. Minteer, 18 : 228-, 2007

12 J. Kim, 24 : 296-, 2006

13 C. Zhou, 4 : 7382-, 2014

14 H. Zhu, 4 : 7333-, 2016

15 M. Christwardana, 299 : 604-, 2015

16 S. C. Barton, 104 : 4867-, 2004

17 E. H. Yu, 3 : 1499-, 2010

18 M. Christwardana, 9 : 1993-, 2017

19 M. Wooten, 86 : 752-, 2014

20 K. Hyun, 40 : 2199-, 2015

21 M. Christwardana, 6 : 3012-, 2016

22 E. -H. Yoo, 10 : 4558-, 2010

23 Y. -M. Uang, 19 : 141-, 2003

24 Y. Liu, 21 : 984-, 2005

25 T. Kong, 138 : 344-, 2009

26 정용진, "새로운 가교제를 적용한 촉매를 이용한 글루코스 센서의 성능향상 연구" 한국화학공학회 53 (53): 802-807, 2015

27 조병훈, "Recent developments and applications of bioinspired silicification" 한국화학공학회 33 (33): 1125-1133, 2016

28 Seungwook Kim, "Production and characterization of cellobiose dehydrogenase from Phanerochaete chrysosporium KCCM 60256 and its application for an enzymatic fuel cell" 한국화학공학회 33 (33): 3434-3441, 2016

29 Shivaji Hariba Pawar, "Immobilization of cellulase on functionalized cobalt ferrite nanoparticles" 한국화학공학회 33 (33): 216-222, 2016

30 World Health Organization (WHO) of United Nations (UN)., "Global Report on Diabetes sheet"

31 Inamuddin, "Fabrication of bioanode by using electrically conducting polythiophene via entrapment technique" 한국화학공학회 33 (33): 120-125, 2016

32 Jian Dong Cui, "A facile technique to prepare cross-linked enzyme aggregates of bovine pancreatic lipase using bovine serum albumin as an additive" 한국화학공학회 33 (33): 610-615, 2016