콜라겐을 알긴산으로 교차 결합시킨 인공진피의 창상치유 효과 = The Wound Healing Effects of Alginate- crosslinked Collagen Dermal Substitute of Artificial Skin

다국어 초록 (Multilingual Abstract)

Alginate, which is isolated from brown seaweed, is a bioabsorbable long chain polysaccharides, β-D-mannuronic acid and α-L-guluronic acid. The authors produced alginates and alginate-colllagen as a disc form. Then, to evaluate the efficacy of alginate and alginate-collagen complex as a wound healing material, three full-thickness skin defects of 2 cm in diameter were made at the back of the New Zealand white rabbits. Three groups of dressing materials-alginate, alginate-collagen complex and vaseline gauze as control group - were applied on the wound and the results were evaluated grossly and histopathologically. The authors compared gross findings of sizes of healed wound, wound epithelization and wound contraction by tracing the remaining wound area at 5th, 10th, 15th, 20th, 25th day after wound introduction, and wound biopsy was performed at 3rd, 7th, 14th, 21st day, respectively.Alginate and alginate-collagen complex showed statistically higher percentage of wound contraction and wound healing compared to control group(p<0.05). Alginate-collagen complex showed statistically higher percentage of wound contraction, epithelization and wound healing compared to alginate alone. In conclusion, the result suggests that alginate has a good effect of wound healing and that alginate-collagen complex is more effective in wound healing than alginate alone.

참고문헌 (Reference)

1 Yoo, "The role of alginate sponge on growth and differentiation of preadipocytes" 28 : 2001

2 Kwon H, "The effect of Poly-L-lysine on proliferation and differentiation of preadipocyte in the alginate gels" 28 : 2001

3 Liu M, "Relationships between nerves and myofibroblasts during cutaneous wound healing in the developing rat" 297 : 1999

4 Shapiro L, "Novel alginate sponges for cell culture and transplantation" 18 : 1997

5 Thie M, "Mechanical confinement inhibits collagen synthesis in gel- cultured fibroblasts" 48 : 294-, 1989

6 Jeong JH, "Injectable cartilage tissue engineering techniques utilizing fibrin glue,liquid alginate and pluronic F-127" 30 : 2003

7 Paige KT, "Injectable cartilage" 96 : 1995

8 Yoo G, "Growth and differentiation of preadipocytes in alginate and collagen gels" 27 : 2000

9 Hilliou F, "Growth and differentiation of 3T3-F442A preadipocytes in three- dimensional gels of native collagen" 177 : 372-, 1988

10 Atala A, "Endoscopic treatment of vesicoureteral reflux with a chondrocyte-alginate suspension" 152 : 641-, 1994

11 Baek RM, "Effects of the Recombinant Human Epidermal Growth factor on Full-thickness Wound of the Rat Skin" 30 : 2003

12 van Susante JL, "Culture of chondrocytes in alginate and collagen carrier gels" 66 : 1995

13 Grandolfo M, "Culture and differentiation of chondrocytes entrapped in alginate gels" 52 : 42-, 1993

14 Smidsrød O, "Alginate as immobilization matrix for cells" 8 : 71-, 1990

15 Halberstadt C, "A hydrogel material for plastic and reconstructive applications injected into the subcutaneous space of a sheep" 8 : 2002