HPLC를 이용한 낙지다리 추출물의 (-)Epicatechin gallate 분석법 개발 = Analytical Method Development of (-)-Epicatechin gallate in Penthorum chinense Pursh Extract using HPLC

국문 초록 (Abstract)

본 연구는 낙지다리(Penthorum chinense Pursh) 추출물을 기능성 화장품소재로 개발하기 위해 (-)-epicatechin gallate를 지표성분으로 선정하고, 품질관리를 위해 High Performance Liquid Chromatography (HPLC)를 이용하여 분석법을 개발하였다. 분석에 사용된 칼럼은 Unison US-C₁₈ (4.6 × 250 mm, 5 μm, Imtakt, USA)을 사용하여 0.05% (v/v) trifluoroacetic acid (TFA)와 메탄올을 이동상 조건으로 컬럼 온도는 30 ℃ 에서 유속은 1.0 mL/min 로 검출파장은 280 nm에서 검출하였다. International Conference on Harmonization (ICH) 가이드라인(version 4, 2005)을 근거로 하여 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계를 분석하여 분석방법을 검증하였다. 검출한계 및 정량한계는 각각 0.11 mg/mL 및 0.33 mg/mL로 나타났으며, 검량곡선은 상관계수값이 0.9999로 양호한 직선성을 보였고, 정밀성 분석결과도 0.6% 이하로 확인하였다. 또한, 회수율은 99.51 ~ 101.92% 범위로 정확성이 있음을 알 수 있다. 따라서, 본 분석법은 낙지다리 추출물의 지표성분의 분석법은 적합한 시험법임이 검증되었다.

다국어 초록 (Multilingual Abstract)

This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison US-C₁₈ column (4.6 × 250 mm, 5 μm) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at 30 ℃. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r² > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

참고문헌 (Reference)

1 장태원, "Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts" 한국자원식물학회 31 (31): 667-674, 2018

2 J. O. Gu, "Water and Ruderal Plant Flora of Korea" Resource Association for Research on Plant Protection 101-, 2010

3 A. Wang, "Traditional chinese herbal medicine Penthorum chinense Pursh: A phytochemical and pharmacological review" 43 (43): 601-, 2015

4 J. K. Jackson, "The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate" 21 (21): 1435-, 2010

5 Ministry of Food and Drug Safety, "The Korean Pharmacopeia" Shinilbooks 1366-, 2008

6 T. Hanamura, "Skin-lightening effect of a polyphenol extract from Acerola(Malpighia emarginata DC. )fruit on uv-induced pigmentation" 72 (72): 3211-, 2008

7 J. H. Chung, "Regulations of collagen synthesis by ascrobic acid, transforming growth factor-β and interferon-γ in human dermal fibroblasts cultured in three-dimensional collagen gel are photoaging- and aging-independent" 15 (15): 188-, 1997

8 Zhe Fang, "Quantitative and Pattern Recognition Analyses for the Quality Evaluation of Magnoliae Flos by HPLC" 대한화학회 31 (31): 3371-3381, 2010

9 NGUYENTHIYEN, "Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography" 대한화학회 35 (35): 1956-1964, 2014

10 H. S. Hwang, "Prospects for development of cosmetic industry using natural products in Chungbuk" 2018

11 D. Huang, "Polyphenols with anti-proliferative activities from Penthorum chinense Pursh" 19 (19): 11045-, 2014

12 Y. C. He, "Penthorin A and B, two unusual 2,4′-epoxy-8,5′-neolignans from Penthorum chinese" 100 : 7-, 2015

13 Q. Lu, "Isolation and identification of compounds from Penthorum chinense Pursh with antioxidant and antihepatocarcinoma properties" 60 (60): 11097-, 2012

14 J. Wittenauer, "Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity" 101 : 179-, 2015

15 O. L. Aruma, "Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivatives" 41 (41): 1880-, 1993

16 G. Dannhardt, "Cyclooxygenase inhibitorscurrent status and future prospects" 36 (36): 109-, 2001

17 S. A. Wiseman, "Antioxidants in Tea" 37 (37): 705-, 1997

18 이경준, "Antioxidant and Biological Activity in the Leaves of Adzuki Bean (Vigna angularis L.)" 한국자원식물학회 31 (31): 237-253, 2018

19 L. Xiang, "Anti-aging effects of phloridzin, an apple polyphenol, on yeast via the SOD and Sir2genes" 75 (75): 854-, 2011

20 M. Wang, "A new flavanone from the aerial parts of Penthorum chinense" 28 (28): 70-, 2014