Eupatilin Ameliorates Collagen Induced Arthritis

다국어 초록 (Multilingual Abstract)

Eupatilin is the main active component of DA-9601, an extract from Artemisia. Recently,eupatilin was reported to have anti-inflammatory properties. We investigated the antiarthriticeffect of eupatilin in a murine arthritis model and human rheumatoidsynoviocytes. DA-9601 was injected into collagen-induced arthritis (CIA) mice. Arthritisscore was regularly evaluated. Mouse monocytes were differentiated into osteoclasts wheneupatilin was added simultaneously. Osteoclasts were stained with tartrate-resistant acidphosphatase and then manually counted. Rheumatoid synoviocytes were stimulated withTNF-α and then treated with eupatilin, and the levels of IL-6 and IL-1β mRNA expression insynoviocytes were measured by RT-PCR. Intraperitoneal injection of DA-9601 reducedarthritis scores in CIA mice. TNF-α treatment of synoviocytes increased the expression ofIL-6 and IL-1β mRNAs, which was inhibited by eupatilin. Eupatilin decreased the numberof osteoclasts in a concentration dependent manner. These findings, showing thateupatilin and DA-9601 inhibited the expression of inflammatory cytokines and thedifferentiation of osteoclasts, suggest that eupatilin and DA-9601 is a candidateanti-inflammatory agent.

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