포장방법에 따른 산약(山藥) 지표성분의 함량분석 = Quantitative Determination of the Marker Constituent of Dioscoreae Rhizoma by the Packaging Methods

다국어 초록 (Multilingual Abstract)

Objectives: To investigate the quantitative determination of marker constituents of Dioscoreae Rhizoma by the packaging methods. Methods: HPLC for the determinations of allatoin in the Dioscoreae Rhizoma, the separation method was performed on an Luna NH2 column (250 mm×4.6 mm, 5 μm, Phenomenes) using solvent water-acetonitrile (2:8, v/v%) with photo diode array detector (210nm). The flow rate was 2.0 mL/min. Results: Retention time of allatoin in HPLC chromatogram was about 4.8 min and calibration curve showed good linearity (R2=0.9994) at concentrations from 50.0 to 1000.0 μg/mL of allantoin. Average content of allantoin by packaging methods was 0.16~0.40%. In addition, weight loss rate (%) of Dioscoreae Rhizoma according to the packaging methods was -0.60~1.80%. Conclusions: The variation on content of the marker constituent of Dioscoreae Rhizoma by the packaging methods didn`t show difference.

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