Combination of mineral trioxide aggregate and propolis promotes odontoblastic differentiation of human dental pulp stem cells through ERK signaling pathway

다국어 초록 (Multilingual Abstract)

The aim of this study is to investigate combinedeffects of mineral trioxide aggregate (MTA) and propolison odontoblastic differentiation of human dental pulp stemcells (DPSCs) and to find a signaling pathway involved.
Combination of MTA and propolis significantly up-regulatedthe expression of DSPP and DMP1, and facilitated amineral nodule formation (p\0.05). Treatments withMTA, propolis or combined increased the phosphorylationof extracellular signal-regulated kinases (ERK), one ofmitogen-activated protein kinases signaling cascades duringodontogenic differentiation of DPSCs (p\0.05), andU0126, an inhibitor of ERK, decreased calcium deposits(p\0.05). Combination of MTA and propolis promotesodontogenic differentiation and mineralization of DPSCsthrough ERK pathway.

참고문헌 (Reference)

1 우수미, "Vitamin D Promotes Odontogenic Differentiation of Human Dental Pulp Cells via ERK Activation" 한국분자세포생물학회 38 (38): 604-609, 2015

2 Jacob A, "Transcriptional regulation of dentin matrix protein 1 (DMP1) in odontoblasts and osteoblasts" 55 : 107-112, 2014

3 Greenblatt MB, "The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice" 120 (120): 2457-2473, 2010

4 Feng JQ, "The Dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development" 82 : 776-780, 2003

5 Gronthos S, "Stem cell properties of human dental pulp stem cells" 81 : 531-535, 2002

6 Nieva Moreno MI, "Screening of antibacterial activity of Amaicha del Valle (Tucumán, Argentina) propolis" 68 : 97-102, 1999

7 Marcucci MC, "Propolis: chemical composition, biological properties and therapeutic activity" 26 : 83-99, 1995

8 Ahangari Z, "Propolis: chemical composition and its applications in endodontics" 13 : 285-292, 2018

9 Pileggi R, "Propolis inhibits osteoclast maturation" 25 : 584-588, 2009

10 Bretz WA, "Preliminary report on the effects of propolis on wound healing in the dental pulp" 53 : 1045-1048, 1998

11 Liu CH, "Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway" 43 : 359-366, 2014

12 Qureshi A, "Nandakumar, Pratapkumar, and Sambashivarao. Recent advances in pulp capping materials: an overview" 8 (8): 316-321, 2014

13 Johnson GL, "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases" 298 : 1911-1912, 2002

14 Zhao X, "Mineral trioxide aggregate promotes odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp stem cells" 39 : 215-220, 2012

15 Huang GT, "Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine" 88 : 792-806, 2009

16 Moreau JL, "Mesenchymal stem cell proliferation and differentiation on an injectable calcium phosphate-chitosan composite scaffold" 30 : 2675-2682, 2009

17 Yang SH, "MAP kinase signaling cascades and transcriptional regulation" 513 : 1-13, 2013

18 Wu Y, "Intermittent traction stretch promotes the osteoblastic differentiation of bone mesenchymal stem cells by the ERK1/2-activated Cbfa1 pathway" 53 (53): 451-459, 2012

19 Lv T, "Insulin-like growth factor 1 promotes the proliferation and committed differentiation of human dental pulp stem cells through MAPK pathways" 72 : 116-123, 2016

20 Hwang YC, "Influence of TGF-beta1 on the expression of BSP, DSP, TGF-beta1 receptor I and Smad proteins during reparative dentinogenesis" 39 : 153-160, 2008

21 George A, "In situ hybridization shows Dmp1 (AG1) to be a developmentally regulated dentin-specific protein produced by mature odontoblasts" 33 : 67-72, 1995

22 Sabir A, "Histological analysis of rat dental pulp tissue capped with propolis" 47 : 135-138, 2005

23 Carrotte P, "Endodontics: Part 9. Calcium hydroxide, root resorption, endo-perio lesions" 197 : 735-743, 2004

24 Hakki SS, "Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts" 35 (35): 513-519, 2009

25 Hwu YJ, "Effectiveness of propolis on oral health: a meta-analysis" 22 : 221-229, 2014

26 Ahangari Z, "Effect of propolis on dentin regeneration and the potential role of dental pulp stem cell in Guinea pigs" 13 : 223-228, 2012

27 Middleton E Jr, "Effect of plant flavonoids on immune and inflammatory cell function" 439 : 175-182, 1998

28 Hilkens P, "Effect of isolation methodology on stem cell properties and multilineage differentiation potential of human dental pulp stem cells" 353 : 65-78, 2013

29 Narayanan K, "Dual functional roles of dentin matrix protein 1. Implications in biomineralization and gene transcription by activation of intracellular Ca2+ store" 278 : 17500-17508, 2003

30 Yamamura T, "Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound healing" 64 : 530-540, 1985

31 Narayanan K, "Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation" 281 : 19064-19071, 2006

32 Shivani Utneja, "Current perspectives of bio-ceramic technology in endodontics: calcium enriched mixture cement - review of its composition, properties and applications" 대한치과보존학회 40 (40): 1-13, 2015

33 Ge C, "Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development" 176 : 709-718, 2007

34 Schwab KE, "Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium" 22 : 2903-2911, 2007

35 Naik RM, "Can MTA be: Miracle trioxide aggregate?" 18 : 5-8, 2014

36 Ang ES, "Caffeic acid phenethyl ester, an active component of honeybee propolis attenuates osteoclastogenesis and bone resorption via the suppression of RANKL-induced NF-kappaB and NFAT activity" 221 : 642-649, 2009

37 Scheller S, "Biological properties and clinical application of propolis. IX, Experimental observation on the influence of ethanol extract of propolis (EEP) on dental pulp regeneration" 28 (28): 289-291, 1978

38 Osés SM, "Bioactive properties of honey with propolis" 196 : 1215-1223, 2016

39 Toker H, "A morphometric and histopathologic evaluation of the effects of propolis on alveolar bone loss in experimental periodontitis in rats" 79 (79): 1089-1094, 2008

40 Raoof M, "A modified efficient method for dental pulp stem cell isolation" 11 : 244-250, 2014

41 Parolia A, "A comparative histological analysis of human pulp following direct pulp capping with Propolis, mineral trioxide aggregate and Dycal" 55 : 59-64, 2010

42 Woo SM, "17beta-estradiol induces odontoblastic differentiation via activation of the c-Src/MAPK pathway in human dental pulp cells" 93 (93): 587-595, 2015